CD326 (EpCAM) Monoclonal Antibody (G8.8), APC-eFluor™ 780, eBioscience™, Invitrogen™

Description

• Description: The G8.8 monoclonal antibody reacts with the 40 kDa protein mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40 (epithelial glycoprotein 40), 17-1A antigen, TACSTD1 (tumor-associated calcium signal transducer 1), and CD326
• The immunogen used to generate the G8.8 antibody was the TE-71 thymic epithelial cell line
• CD326 is expressed on the majority of epithelial cells, and is considered a pan-carcinoma antigen
• CD326 mediates calcium-independent, homophilic, cell-cell adhesion and may function as a growth factor receptor
• The antigen is being used as a target for immunotherapy treatment of human carcinomas
• CD326 binds LAIR-1 (CD305) and LAIR-2 (CD306) to inhibit cellular activation and inflammation
• This epithelial glycoprotein is now recognized as having an important role in tumor biology
• Applications Reported: This G8.8 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This G8.8 antibody has been tested by flow cytometric analysis of the TE-71 cell line
• This can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule
• Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E
• Ep-CAM mediates Ca2+-independent homotypic interactions
• Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells
• Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation
• Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage
• Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types
• Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.