CD14 Monoclonal Antibody (61D3), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein
• CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells
• Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14
• Applications Reported: This 61D3 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• eFluor™ 450 is an alternative to Pacific Blue™
• eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm)
• Please make sure that your instrument is capable of detecting this fluorochrome
• Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils
• CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form
• CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein
• The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles
• Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes
• Alternative splicing results in multiple transcript variants encoding the same CD14 isoform
• Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.