CD23 Monoclonal Antibody (B3B4), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein
• CD23 is expressed on resting conventional B cells, and its expression is modulated upon B-cell activation
• B-1 cell lineage (CD5+ B cells) does not express CD23
• Soluble forms of the antigen have been reported to be biologically active
• CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B-cell activation
• CD21 is thought to bind to CD23
• Applications Reported: This B3B4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This B3B4 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor™ 450 is an alternative to Pacific Blue™
• eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts
• The CD23 molecule is identical to the low affinity IgE receptor found on B cells
• Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma
• CD23 is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts
• Functionally, CD23 is involved in B cell growth and differentiation, and IgE production
• Further, CD23 has a soluble form that is a potent mitogenic factor
• Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.