IL-1 beta (Pro-form) Monoclonal Antibody (NJTEN3), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: This NJTEN3 monoclonal antibody reacts with the pro-form of mouse IL-1 beta, which is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells
• It is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol
• Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals
• Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta
• IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is instead secreted alongside caspase-1 via an alternate and incompletely understood mechanism
• IL-1 beta signals via the IL-1RI, which is shared with IL-1 alpha
• These cytokines play important roles in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses
• Their activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition
• Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions, giving IL-RA or IL-1 beta neutralizing antibodies potential therapeutical value
• Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells
• It is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol
• Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals
• Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta
• IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is instead secreted alongside caspase-1 via an alternate and incompletely understood mechanism
• Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions.IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha
• These cytokines play important roles in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses
• Their activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition
• Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions, giving IL-RA or IL-1 beta neutralizing antibodies potential therapeutical value.