7091641

IL-13 Monoclonal Antibody (eBio13A), PE-eFluor™ 610, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

IL-13

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

ALRH; ALRH antibody; BHR1; il 13; Il13; Il-13; IL-13; cytokine; ILN; Interleukin; interleukin 13; Interleukin13; interleukin-13; interleukin-13 precursor; L13; MGC116786; MGC116788; MGC116789; NC30; NC30 antibody; P600; P600 antibody; T-cell activation protein P600

Gene Symbols

Il13

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

Il13

Clone

eBio13A

Applications

Flow Cytometry

Conjugate

PE-eFluor 610

Host Species

Rat

Target Species

Mouse

Gene Accession No.

P20109

Gene ID (Entrez)

16163

Isotype

IgG1 κ

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: The eBio13A antibody reacts with mouse IL-13
  • IL-13 is a cytokine produced mainly by Th2 cells, but also by antigen-primed CD8 T cells
  • IL-13 has a strong involvement in allergic inflammation and parasitic clearing
  • In cancer models it has been shown to have either inhibitory or stimulatory activity depending on the tumor
  • In humans, IL-13 is found to play a role in isotype switching in B cells
  • IL-13 is implicating in down modulating macrophage activity, through the reduction of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12) Applications Reported: This eBio13A antibody has been reported for use in intracellular staining followed by flow cytometric analysis
  • Applications Tested: This eBio13A antibody has been tested by intracellular staining and flow cytometric analysis of Th2-polarized mouse splenocytes
  • This can be used at less than or equal to 1 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • PE-eFluor™ 610 can be excited with laser lines from 488-561 nm and emits at 607 nm
  • We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red™)
  • IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses
  • IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils
  • IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells
  • Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF
  • The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

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Antigen:
IL-13

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
ALRH; ALRH antibody; BHR1; il 13; Il13; Il-13; IL-13; cytokine; ILN; Interleukin; interleukin 13; Interleukin13; interleukin-13; interleukin-13 precursor; L13; MGC116786; MGC116788; MGC116789; NC30; NC30 antibody; P600; P600 antibody; T-cell activation protein P600

Gene Symbols:
Il13

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Il13

Clone:
eBio13A

Applications:
Flow Cytometry

Conjugate:
PE-eFluor 610

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P20109

Gene ID (Entrez):
16163

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
IFN gamma

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g

Gene Symbols:
IFNG

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
IFNG

Clone:
XMG1.2

Applications:
Flow Cytometry

Conjugate:
PE-eFluor 610

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P01580

Gene ID (Entrez):
15978

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
Ly-6G

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
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Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
Gr1; Gr-1; Ly6g; Ly-6G; ly-6G.1; lymphocyte antigen 6 complex, locus G; lymphocyte antigen 6G

Gene Symbols:
Ly6g

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Ly6g

Clone:
1A8-Ly6g

Applications:
Flow Cytometry

Conjugate:
PE-eFluor 610

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P35461

Gene ID (Entrez):
546644

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
HELIOS

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
A730095J18Rik; ANF1A2; Helios; IKAROS family zinc finger 2; IKAROS family zinc finger 2 (Helios); ikaros family zinc finger protein 2; Ikzf2; Zfpn1a2; zinc finger DNA binding protein Helios; zinc finger protein Helios; zinc finger protein, subfamily 1A, 2 (Helios); ZNF1A2; Znfn1a2

Gene Symbols:
IKZF2

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
IKZF2

Clone:
22F6

Applications:
Flow Cytometry

Conjugate:
PE-eFluor 610

Host Species:
Armenian Hamster

Target Species:
Canine, Human, Mouse

Gene Accession No.:
P81183, Q9UKS7

Gene ID (Entrez):
22779, 22807, 488510

Isotype:
IgG

Purification Method:
Affinity chromatography

Product Type:
Antibody