IL-10 Monoclonal Antibody (JES3-9D7), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10)
• Applications Reported: This JES3-9D7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This JES3-9D7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Bufer Set (cat
• 88-8824) and protocol
• Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic)intracellular proteins
• This can be used at 5 μL (0.03 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
• We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
• Please make sure that your instrument is capable of detecting this fluorochrome
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes
• This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages
• It also enhances B cell survival, proliferation, and antibody production
• Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway
• Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract
• Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis
• Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.