CD16 Mouse anti-Human, PE-Cy5, Clone: 3G8, BD

Description

• The 3G8 monoclonal antibody specifically binds to the 50-65 kDa transmembrane form of the IgG Fc Receptor (FcγRIII), a human NK cell-associated antigen
• CD16 is expressed on NK cells as well as macrophages and granulocytes
• Reports indicate that CD16 plays a role in signal transduction and NK cell activation
• The 3G8 antibody blocks the binding of soluble immune complexes to granulocytes
• The 3G8 antibody is reported (Vossebeld et al ., 1997) to increase intracellular calcium levels in human neutrophils by interacting with bothFcγRIIa and FcγRIIIb molecules
• This antibody has also been reported to induce homotypic neutrophil aggregation
• This clone also cross-reacts with a subset of peripheral blood lymphocytes and monocytes, but not granulocytes, of baboon and both rhesus and cynomologus macaque monkeys
• Multi-color analysis reveals that the distribution on lymphocytes is similar to that found in human studies with the majority of CD16-positive lymphocytes being both CD3 and CD20 negative.