7092525

CD3 Mouse anti-Human, APC, Clone: UCHT1, BD

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD3

Applications

Flow Cytometry

Conjugate

APC

Regulatory Status

RUO

Formulation

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype

IgG1 κ

Purification Method

Affinity Purified

Clone

UCHT1

Classification

Monoclonal

Host Species

Mouse

Target Species

Human

Immunogen

Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Description

  • The UCHT1 monoclonal antibody specifically binds to the human CD3ε-chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex
  • CD3ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes
  • Studies from the HLDA Workshop show that this antibody is mitogenic for CD3ε-positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody
  • CD3 plays a central role in signal transduction during antigen recognition
  • The UCHT1 antibody stains both surface and intracellular CD3ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3ε.

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Show Difference

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Antigen:
CD3

Applications:
Flow Cytometry

Conjugate:
APC

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Clone:
UCHT1

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Immunogen:
Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Antigen:
CD3

Applications:
Flow Cytometry

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Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype:
IgG2a κ

Purification Method:
Affinity Purified

Clone:
HIT3A

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Immunogen:
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Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype:
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Purification Method:
Affinity Purified

Clone:
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Classification:
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Host Species:
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Target Species:
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Antigen:
CD3

Applications:
Flow Cytometry

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Regulatory Status:
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Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Isotype:
IgM κ

Purification Method:
Affinity Purified

Clone:
1F4

Classification:
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Host Species:
Mouse

Target Species:
Rat

Immunogen:
F344 rat splenic T cells stimulated with PMA plus A23187

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.