7092736

HLA-DR Mouse anti-Human, V500, Clone: G46-6, BD

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

HLA-DR

Applications

Flow Cytometry

Conjugate

Violet Fluor 500 (V500)

Regulatory Status

RUO

Formulation

Aqueous buffered solution containing protein stabilizer, glycerol and ≤0.09% sodium azide.

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone

G46-6

Classification

Monoclonal

Host Species

Mouse

Target Species

Human

Isotype

IgG2a κ

Purification Method

Affinity Purified

Description

MHC class II antigen; HLA class II histocompatibility antigen

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Description

  • The G46-6 monoclonal antibody specifically binds to HLA-DR, a major histocompatibility complex (MHC) class II antigen
  • HLA-DR antigens are encoded by genes within the Human Leukocyte Antigen (HLA) Complex located on chromosome 6
  • HLA-DR is a transmembrane heterodimeric glycoprotein composed of an α chain (36 kDa) and a β subunit (27 kDa) expressed primarily on antigen presenting cells: B cells, dendritic cells, monocytes, macrophages, and thymic epithelial cells
  • HLA-DR is also expressed on activated T cells
  • This molecule plays a major role in mediating cellular interactions during antigen presentation to CD4-positive T cells
  • When compensating dyes in this spectral range (such as Horizon™ V500 and AmCyan), the most accurate compensation can be obtained using single stained cellular controls
  • Due to spectral differences between cells and beadsin this channel, using BD CompBeads can result in spillover errors for V500 and AmCyan reagents.Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended.Different V500 reagents (e.g
  • CD4 vs
  • CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.

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Antigen:
HLA-DR

Applications:
Flow Cytometry

Conjugate:
Violet Fluor 500 (V500)

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing protein stabilizer, glycerol and ≤0.09% sodium azide.

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
G46-6

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Isotype:
IgG2a κ

Purification Method:
Affinity Purified

Description:
MHC class II antigen; HLA class II histocompatibility antigen

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Antigen:
I-A/I-E

Applications:
Flow Cytometry

Conjugate:
FITC

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
2G9

Classification:
Monoclonal

Host Species:
Rat

Target Species:
Mouse

Isotype:
IgG2a κ

Purification Method:
Affinity Purified

Description:
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Antigen:
IFN-γ

Applications:
Flow Cytometry

Conjugate:
PE

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
B27

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Description:
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Antigen:
IFN-γ

Applications:
Flow Cytometry

Conjugate:
APC

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
XMG1.2

Classification:
Monoclonal

Host Species:
Rat

Target Species:
Mouse

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Description:
__