ATG12 Recombinant Polyclonal Antibody (20 HCLC), Invitrogen™

Description

• This antibody is predicted to react with Monkey, Pig and Horse
• Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific
• They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody
• While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains
• The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production
• ATG12, a member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy
• This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells
• ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7
• At least two isoforms of ATG12 are known to exist
• Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells
• It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components
• This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1.