7105821

NF-H Antibody (SPM203) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

NF-H

Concentration

1.0 mg/mL

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), CyTOF

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Autophagy, Cellular Markers, Cytoskeleton Markers, Neurodegeneration, Neurofilaments, Neuronal Cell Markers, Neuroscience

Formulation

PBS with No Preservative

Gene ID (Entrez)

4744

Immunogen

Triton-X 100 insoluble protein fraction of rat brain

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen

200 kDa

Clone

SPM203

Dilution

Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, CyTOF-ready

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Porcine, Chicken

Gene Alias

200 kDa neurofilament protein, KIAA0845, Neurofilament Heavy (200kDa), neurofilament heavy polypeptide, Neurofilament triplet H protein, neurofilament, heavy polypeptide, neurofilament, heavy polypeptide 200kDa, NF200, NFH, NF-H

Gene Symbols

NEFH

Isotype

IgG1

Purification Method

Protein A or G purified

Test Specificity

This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

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Description

  • NF-H Monoclonal specifically detects NF-H in Human, Mouse, Rat, Porcine, Chicken samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, CyTOF-ready.

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Fischer Scientific

7105821

Mouse Monoclonal Antibody...


Antigen:
NF-H

Concentration:
1.0 mg/mL

Applications:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), CyTOF

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Autophagy, Cellular Markers, Cytoskeleton Markers, Neurodegeneration, Neurofilaments, Neuronal Cell Markers, Neuroscience

Formulation:
PBS with No Preservative

Gene ID (Entrez):
4744

Immunogen:
Triton-X 100 insoluble protein fraction of rat brain

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
200 kDa

Clone:
SPM203

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, CyTOF-ready

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Porcine, Chicken

Gene Alias:
200 kDa neurofilament protein, KIAA0845, Neurofilament Heavy (200kDa), neurofilament heavy polypeptide, Neurofilament triplet H protein, neurofilament, heavy polypeptide, neurofilament, heavy polypeptide 200kDa, NF200, NFH, NF-H

Gene Symbols:
NEFH

Isotype:
IgG1

Purification Method:
Protein A or G purified

Test Specificity:
This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Img

Fischer Scientific

7105822

Mouse Monoclonal Antibody...


Antigen:
NF-H

Concentration:
1.0 mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), CyTOF

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Autophagy, Cellular Markers, Cytoskeleton Markers, Neurodegeneration, Neurofilaments, Neuronal Cell Markers, Neuroscience

Formulation:
PBS with No Preservative

Gene ID (Entrez):
4744

Immunogen:
Crude neurofilament preparation from porcine spinal cord

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
200 kDa

Clone:
NE14

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, CyTOF-ready

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Porcine, Chicken, Feline, Gerbil, Guinea Pig, Primate, Rabbit

Gene Alias:
200 kDa neurofilament protein, KIAA0845, Neurofilament Heavy (200kDa), neurofilament heavy polypeptide, Neurofilament triplet H protein, neurofilament, heavy polypeptide, neurofilament, heavy polypeptide 200kDa, NF200, NFH, NF-H

Gene Symbols:
NEFH

Isotype:
IgG1

Purification Method:
Protein A or G purified

Test Specificity:
This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

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7105824

Mouse Monoclonal Antibody...


Antigen:
Ornithine Decarboxylase

Concentration:
1.0 mg/mL

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cell Cycle and Replication

Formulation:
PBS with No Preservative

Gene ID (Entrez):
4953

Immunogen:
Recombinant human ODC-1 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
53 kDa

Clone:
ODC1/486

Dilution:
Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Rat

Gene Alias:
ODCEC 4.1.1.17, ornithine decarboxylase, ornithine decarboxylase 1

Gene Symbols:
ODC1

Isotype:
IgG2a κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.

Img

Fischer Scientific

7105824

Mouse Monoclonal Antibody...


Antigen:
Ornithine Decarboxylase

Concentration:
1.0 mg/mL

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cell Cycle and Replication

Formulation:
PBS with No Preservative

Gene ID (Entrez):
4953

Immunogen:
Recombinant human ODC-1 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
53 kDa

Clone:
ODC1/486

Dilution:
Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Rat

Gene Alias:
ODCEC 4.1.1.17, ornithine decarboxylase, ornithine decarboxylase 1

Gene Symbols:
ODC1

Isotype:
IgG2a κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.