7105918
G-CSF Antibody (SPM468) - Azide and BSA Free, Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
G-CSF
Concentration
1.0 mg/mL
Applications
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cell Cycle and Replication, Cytokine Research
Formulation
PBS with No Preservative
Gene ID (Entrez)
1440
Immunogen
Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen
19 kDa
Clone
SPM468
Dilution
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Monkey
Gene Alias
C17orf33, chromosome 17 open reading frame 33, colony stimulating factor 3 (granulocyte), CSF3OS, filgrastim, G-CSF, GCSFlenograstim, granulocyte colony-stimulating factor, Lenograstim, MGC45931, pluripoietin
Gene Symbols
CSF3
Isotype
IgG1
Purification Method
Protein A or G purified
Test Specificity
This MAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF s, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF s share 73% sequence identity at the amino acid level.
Description
- G-CSF Monoclonal specifically detects G-CSF in Human, Monkey, Rhesus Macaque samples
- It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.
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Antigen: G-CSF
Concentration: 1.0 mg/mL
Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Cycle and Replication, Cytokine Research
Formulation: PBS with No Preservative
Gene ID (Entrez): 1440
Immunogen: Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 19 kDa
Clone: SPM468
Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Monkey
Gene Alias: C17orf33, chromosome 17 open reading frame 33, colony stimulating factor 3 (granulocyte), CSF3OS, filgrastim, G-CSF, GCSFlenograstim, granulocyte colony-stimulating factor, Lenograstim, MGC45931, pluripoietin
Gene Symbols: CSF3
Isotype: IgG1
Purification Method: Protein A or G purified
Test Specificity: This MAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF s, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF s share 73% sequence identity at the amino acid level.
Antigen:
G-CSF
Concentration:
1.0 mg/mL
Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Cycle and Replication, Cytokine Research
Formulation:
PBS with No Preservative
Gene ID (Entrez):
1440
Immunogen:
Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen:
19 kDa
Clone:
SPM468
Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Monkey
Gene Alias:
C17orf33, chromosome 17 open reading frame 33, colony stimulating factor 3 (granulocyte), CSF3OS, filgrastim, G-CSF, GCSFlenograstim, granulocyte colony-stimulating factor, Lenograstim, MGC45931, pluripoietin
Gene Symbols:
CSF3
Isotype:
IgG1
Purification Method:
Protein A or G purified
Test Specificity:
This MAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF s, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF s share 73% sequence identity at the amino acid level.
Antigen: CD63
Concentration: 1.0 mg/mL
Applications: Flow Cytometry, Immunofluorescence, CyTOF
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Autophagy, Cancer, Cardiovascular Biology, Cellular Signaling, Cytokine Research, Immunology, Innate Immunity, Lysosome Markers
Formulation: PBS with No Preservative
Gene ID (Entrez): 967
Immunogen: Recombinant human CD63 protein
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: __
Clone: LAMP3/529
Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: CD63 antigen, CD63 antigen (melanoma 1 antigen), CD63 molecule, Granulophysin, LAMP-3, Lysosomal-associated membrane protein 3, ME491, melanoma 1 antigen, Melanoma-associated antigen ME491, melanoma-associated antigen MLA1, MLA1lysosome-associated membrane glycoprotein 3, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, tspan-30, TSPAN30granulophysin
Gene Symbols: CD63
Isotype: IgG2a κ
Purification Method: Protein A or G purified
Test Specificity: This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/803 or LAMP3/968 or NKI/C3 or MX-49.129.5. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Antigen:
CD63
Concentration:
1.0 mg/mL
Applications:
Flow Cytometry, Immunofluorescence, CyTOF
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Autophagy, Cancer, Cardiovascular Biology, Cellular Signaling, Cytokine Research, Immunology, Innate Immunity, Lysosome Markers
Formulation:
PBS with No Preservative
Gene ID (Entrez):
967
Immunogen:
Recombinant human CD63 protein
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen:
__
Clone:
LAMP3/529
Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
CD63 antigen, CD63 antigen (melanoma 1 antigen), CD63 molecule, Granulophysin, LAMP-3, Lysosomal-associated membrane protein 3, ME491, melanoma 1 antigen, Melanoma-associated antigen ME491, melanoma-associated antigen MLA1, MLA1lysosome-associated membrane glycoprotein 3, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, tspan-30, TSPAN30granulophysin
Gene Symbols:
CD63
Isotype:
IgG2a κ
Purification Method:
Protein A or G purified
Test Specificity:
This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/803 or LAMP3/968 or NKI/C3 or MX-49.129.5. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Antigen: CD63
Concentration: 1.0 mg/mL
Applications: Flow Cytometry, Immunofluorescence, CyTOF
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Autophagy, Cancer, Cardiovascular Biology, Cellular Signaling, Cytokine Research, Immunology, Innate Immunity, Lysosome Markers
Formulation: PBS with No Preservative
Gene ID (Entrez): 967
Immunogen: Recombinant human CD63 protein
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: __
Clone: LAMP3/529
Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: CD63 antigen, CD63 antigen (melanoma 1 antigen), CD63 molecule, Granulophysin, LAMP-3, Lysosomal-associated membrane protein 3, ME491, melanoma 1 antigen, Melanoma-associated antigen ME491, melanoma-associated antigen MLA1, MLA1lysosome-associated membrane glycoprotein 3, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, tspan-30, TSPAN30granulophysin
Gene Symbols: CD63
Isotype: IgG2a κ
Purification Method: Protein A or G purified
Test Specificity: This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/803 or LAMP3/968 or NKI/C3 or MX-49.129.5. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Antigen:
CD63
Concentration:
1.0 mg/mL
Applications:
Flow Cytometry, Immunofluorescence, CyTOF
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Autophagy, Cancer, Cardiovascular Biology, Cellular Signaling, Cytokine Research, Immunology, Innate Immunity, Lysosome Markers
Formulation:
PBS with No Preservative
Gene ID (Entrez):
967
Immunogen:
Recombinant human CD63 protein
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen:
__
Clone:
LAMP3/529
Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
CD63 antigen, CD63 antigen (melanoma 1 antigen), CD63 molecule, Granulophysin, LAMP-3, Lysosomal-associated membrane protein 3, ME491, melanoma 1 antigen, Melanoma-associated antigen ME491, melanoma-associated antigen MLA1, MLA1lysosome-associated membrane glycoprotein 3, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, tspan-30, TSPAN30granulophysin
Gene Symbols:
CD63
Isotype:
IgG2a κ
Purification Method:
Protein A or G purified
Test Specificity:
This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/803 or LAMP3/968 or NKI/C3 or MX-49.129.5. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Antigen: CD63
Concentration: 1.0 mg/mL
Applications: Flow Cytometry, Immunofluorescence, CyTOF
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Autophagy, Cancer, Cardiovascular Biology, Cellular Signaling, Cytokine Research, Immunology, Innate Immunity, Lysosome Markers
Formulation: PBS with No Preservative
Gene ID (Entrez): 967
Immunogen: Recombinant human CD63 protein
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: __
Clone: LAMP3/529
Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: CD63 antigen, CD63 antigen (melanoma 1 antigen), CD63 molecule, Granulophysin, LAMP-3, Lysosomal-associated membrane protein 3, ME491, melanoma 1 antigen, Melanoma-associated antigen ME491, melanoma-associated antigen MLA1, MLA1lysosome-associated membrane glycoprotein 3, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, tspan-30, TSPAN30granulophysin
Gene Symbols: CD63
Isotype: IgG2a κ
Purification Method: Protein A or G purified
Test Specificity: This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/803 or LAMP3/968 or NKI/C3 or MX-49.129.5. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Antigen:
CD63
Concentration:
1.0 mg/mL
Applications:
Flow Cytometry, Immunofluorescence, CyTOF
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Autophagy, Cancer, Cardiovascular Biology, Cellular Signaling, Cytokine Research, Immunology, Innate Immunity, Lysosome Markers
Formulation:
PBS with No Preservative
Gene ID (Entrez):
967
Immunogen:
Recombinant human CD63 protein
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen:
__
Clone:
LAMP3/529
Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
CD63 antigen, CD63 antigen (melanoma 1 antigen), CD63 molecule, Granulophysin, LAMP-3, Lysosomal-associated membrane protein 3, ME491, melanoma 1 antigen, Melanoma-associated antigen ME491, melanoma-associated antigen MLA1, MLA1lysosome-associated membrane glycoprotein 3, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, tspan-30, TSPAN30granulophysin
Gene Symbols:
CD63
Isotype:
IgG2a κ
Purification Method:
Protein A or G purified
Test Specificity:
This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/803 or LAMP3/968 or NKI/C3 or MX-49.129.5. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.