7105954

NF-L Antibody (NR-4) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

NF-L

Concentration

1.0 mg/mL

Applications

Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Autophagy, Cellular Markers, Cytoskeleton Markers, Neurodegeneration, Neurofilaments, Neuronal Cell Markers, Neuroscience

Formulation

PBS with No Preservative

Gene ID (Entrez)

4747

Immunogen

Crude neurofilament preparation from porcine spinal cord

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen

68 kDa

Clone

NR-4

Dilution

Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Rat, Porcine, Chicken, Primate

Gene Alias

68 kDa neurofilament protein, neurofilament protein, light chain, neurofilament subunit NF-L, Neurofilament triplet L protein, neurofilament, light polypeptide, neurofilament-light, NF68FLJ53642, NF-L, NFLlight polypeptide 68kDa

Gene Symbols

NEFL

Isotype

IgG1

Purification Method

Protein A or G purified

Test Specificity

This MAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Description

  • NF-L Monoclonal specifically detects NF-L in Human, Rat, Porcine, Bovine, Chicken samples
  • It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.

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Img

Fischer Scientific

7105954

Mouse Monoclonal Antibody...


Antigen:
NF-L

Concentration:
1.0 mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Autophagy, Cellular Markers, Cytoskeleton Markers, Neurodegeneration, Neurofilaments, Neuronal Cell Markers, Neuroscience

Formulation:
PBS with No Preservative

Gene ID (Entrez):
4747

Immunogen:
Crude neurofilament preparation from porcine spinal cord

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
68 kDa

Clone:
NR-4

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Rat, Porcine, Chicken, Primate

Gene Alias:
68 kDa neurofilament protein, neurofilament protein, light chain, neurofilament subunit NF-L, Neurofilament triplet L protein, neurofilament, light polypeptide, neurofilament-light, NF68FLJ53642, NF-L, NFLlight polypeptide 68kDa

Gene Symbols:
NEFL

Isotype:
IgG1

Purification Method:
Protein A or G purified

Test Specificity:
This MAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Img

Fischer Scientific

7105955

Mouse Monoclonal Antibody...


Antigen:
NF-L

Concentration:
1.0 mg/mL

Applications:
Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Autophagy, Cellular Markers, Cytoskeleton Markers, Neurodegeneration, Neurofilaments, Neuronal Cell Markers, Neuroscience

Formulation:
PBS with No Preservative

Gene ID (Entrez):
4747

Immunogen:
Recombinant human NEFL protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
68 kDa

Clone:
NFL/736

Dilution:
Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 1 - 2 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Rat, Porcine, Chicken, Primate

Gene Alias:
68 kDa neurofilament protein, neurofilament protein, light chain, neurofilament subunit NF-L, Neurofilament triplet L protein, neurofilament, light polypeptide, neurofilament-light, NF68FLJ53642, NF-L, NFLlight polypeptide 68kDa

Gene Symbols:
NEFL

Isotype:
IgG1

Purification Method:
Protein A or G purified

Test Specificity:
This MAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Img

Fischer Scientific

7105956

Mouse Monoclonal Antibody...


Antigen:
CEACAM5/CD66e

Concentration:
1.0 mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Cellular Markers, Immunology

Formulation:
PBS with No Preservative

Gene ID (Entrez):
1048

Immunogen:
Purified human CEA protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
__

Clone:
C66/1260

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 1 - 2 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
Carcinoembryonic antigen, carcinoembryonic antigen-related cell adhesion molecule 5, CD66e antigen, CEACD66e, DKFZp781M2392, Meconium antigen 100

Gene Symbols:
CEACAM5

Isotype:
IgG2a κ

Purification Method:
Protein A or G purified

Test Specificity:
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb reacts with nonspecific cross-reacting antigen (NCA). It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

Img

Fischer Scientific

7105958

Mouse Monoclonal Antibody...


Antigen:
CEACAM5/CD66e

Concentration:
1.0 mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Cellular Markers, Immunology

Formulation:
PBS with No Preservative

Gene ID (Entrez):
1048

Immunogen:
Recombinant full-length human CEA protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
__

Clone:
C66/195

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 1 - 2 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Monkey

Gene Alias:
Carcinoembryonic antigen, carcinoembryonic antigen-related cell adhesion molecule 5, CD66e antigen, CEACD66e, DKFZp781M2392, Meconium antigen 100

Gene Symbols:
CEACAM5

Isotype:
IgG1 κ

Purification Method:
Protein A or G purified

Test Specificity:
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb reacts with nonspecific cross-reacting antigen (NCA) and shows a cross-reaction with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix