Rabbit Monoclonal Antibody
Manufacturer: Fischer Scientific
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CD25/IL-2 R alpha
Western Blot 1:100 - 1:2000, Flow Cytometry 1:10 - 1:1000, Immunohistochemistry 1:10 - 1:500, Blocking/Neutralizing 50 - 200 molar excess, Immunofluorescence 1:10 - 1:500, CyTOF-ready
Monoclonal
Purified
RUO
Human, Mouse, Rat
CD25, CD25 antigen, IDDM10, IL-2 receptor subunit alpha, IL2R, IL-2R subunit alpha, IL-2-RA, IL2-RA, interleukin 2 receptor, alpha, interleukin-2 receptor subunit alpha, p55, TAC antigen, TCGFR
IL2RA
IgG
Protein A or G purified
Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit - binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R ( alpha beta yc) - T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
Basiliximab
Western Blot, Flow Cytometry, Immunohistochemistry, Blocking Assay, Immunofluorescence, CyTOF
Unconjugated
Rabbit
Adaptive Immunity, Apoptosis, B Cell Development and Differentiation Markers, Cytokine Research, Diabetes Research, Immunology, Innate Immunity, Mast Cell Markers, Signal Transduction
PBS with 0.02% Proclin 300. with 0.02% Proclin 300
3559
CD25
Primary
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.