7106107
TLR4 Antibody (HTA125) - Azide and BSA Free, Novus Biologicals™
Mouse Monoclonal Antibody has been used in 26 publications
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
TLR4
Dilution
Western Blot reported in scientific literature (PMID 28546218), Flow Cytometry 1ul/1 million cells, Immunocytochemistry/Immunofluorescence 1:10 - 1:2000. Use reported in scientific literature (Schneeman et al (2005)), Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature (Shimazu et al (1999)), Immunoblotting reported in scientific literature (PMID 19195606), In vitro assay reported in scientific literature (PMID 24512642), Flow (Cell Surface) 2-5 ug/1 million cells, Flow (Intracellular), CyTOF-ready, Block/Neutralize reported in scientific literature (PMID 24512642)
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse, Canine
Gene Alias
ARMD10, CD284, CD284 antigen, hTollhomolog of Drosophila toll, TOLL, toll-like receptor 4
Gene Symbols
TLR4
Isotype
IgG2a
Purification Method
Protein G purified
Test Specificity
The has been shown to block the activation of monocytes with LPS (Paik, et al, 2003).
Clone
HTA125
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunoblot, In vitro Assay
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Adaptive Immunity, Cytokine Research, Immunology, Innate Immunity, Signal Transduction
Gene Accession No.
O00206
Gene ID (Entrez)
7099
Immunogen
This antibody was developed by immunizing mice with Ba/F3 cell line expressing human TLR4 cell surface antigen.
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Description
- TLR4 Monoclonal specifically detects TLR4 in Human, Mouse, Canine samples
- It is validated for Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunoblotting, In vitro assay, Flow (Cell Surface), Flow (Intracellular), CyTOF-ready, Block/Neutralize.
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Antigen: TLR4
Dilution: Western Blot reported in scientific literature (PMID 28546218), Flow Cytometry 1ul/1 million cells, Immunocytochemistry/Immunofluorescence 1:10 - 1:2000. Use reported in scientific literature (Schneeman et al (2005)), Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature (Shimazu et al (1999)), Immunoblotting reported in scientific literature (PMID 19195606), In vitro assay reported in scientific literature (PMID 24512642), Flow (Cell Surface) 2-5 ug/1 million cells, Flow (Intracellular), CyTOF-ready, Block/Neutralize reported in scientific literature (PMID 24512642)
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Canine
Gene Alias: ARMD10, CD284, CD284 antigen, hTollhomolog of Drosophila toll, TOLL, toll-like receptor 4
Gene Symbols: TLR4
Isotype: IgG2a
Purification Method: Protein G purified
Test Specificity: The has been shown to block the activation of monocytes with LPS (Paik, et al, 2003).
Clone: HTA125
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunoblot, In vitro Assay
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Adaptive Immunity, Cytokine Research, Immunology, Innate Immunity, Signal Transduction
Gene Accession No.: O00206
Gene ID (Entrez): 7099
Immunogen: This antibody was developed by immunizing mice with Ba/F3 cell line expressing human TLR4 cell surface antigen.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen:
TLR4
Dilution:
Western Blot reported in scientific literature (PMID 28546218), Flow Cytometry 1ul/1 million cells, Immunocytochemistry/Immunofluorescence 1:10 - 1:2000. Use reported in scientific literature (Schneeman et al (2005)), Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature (Shimazu et al (1999)), Immunoblotting reported in scientific literature (PMID 19195606), In vitro assay reported in scientific literature (PMID 24512642), Flow (Cell Surface) 2-5 ug/1 million cells, Flow (Intracellular), CyTOF-ready, Block/Neutralize reported in scientific literature (PMID 24512642)
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Canine
Gene Alias:
ARMD10, CD284, CD284 antigen, hTollhomolog of Drosophila toll, TOLL, toll-like receptor 4
Gene Symbols:
TLR4
Isotype:
IgG2a
Purification Method:
Protein G purified
Test Specificity:
The has been shown to block the activation of monocytes with LPS (Paik, et al, 2003).
Clone:
HTA125
Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunoblot, In vitro Assay
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Adaptive Immunity, Cytokine Research, Immunology, Innate Immunity, Signal Transduction
Gene Accession No.:
O00206
Gene ID (Entrez):
7099
Immunogen:
This antibody was developed by immunizing mice with Ba/F3 cell line expressing human TLR4 cell surface antigen.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen: CD79A
Dilution: Flow Cytometry 1:50-1:100, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1:500, Immunohistochemistry-Frozen 1:10-1:500
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Equine, Guinea Pig, Goat, Ferret, Monkey, Rabbit
Gene Alias: CD79a antigen, CD79A antigen (immunoglobulin-associated alpha), CD79a molecule, immunoglobulin-associated alpha, IGAB-cell antigen receptor complex-associated protein alpha chain, Ig-alpha, MB1, MB-1, MB-1 membrane glycoprotein, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein
Gene Symbols: CD79A
Isotype: IgG1
Purification Method: Protein G purified
Test Specificity: NB100-64347 recognizes an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45kD protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signalling following antigen-binding to surface immunoglobulin. Clone HM57 has been reported to work in western blotting applications.
Clone: HM57
Applications: Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Adaptive Immunity, Cell Biology, Immunology
Gene Accession No.: P11912
Gene ID (Entrez): 973
Immunogen: Synthetic peptide corresponding to 202-216 amino acid sequence of human mb-1.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen:
CD79A
Dilution:
Flow Cytometry 1:50-1:100, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1:500, Immunohistochemistry-Frozen 1:10-1:500
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Equine, Guinea Pig, Goat, Ferret, Monkey, Rabbit
Gene Alias:
CD79a antigen, CD79A antigen (immunoglobulin-associated alpha), CD79a molecule, immunoglobulin-associated alpha, IGAB-cell antigen receptor complex-associated protein alpha chain, Ig-alpha, MB1, MB-1, MB-1 membrane glycoprotein, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein
Gene Symbols:
CD79A
Isotype:
IgG1
Purification Method:
Protein G purified
Test Specificity:
NB100-64347 recognizes an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45kD protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signalling following antigen-binding to surface immunoglobulin. Clone HM57 has been reported to work in western blotting applications.
Clone:
HM57
Applications:
Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Adaptive Immunity, Cell Biology, Immunology
Gene Accession No.:
P11912
Gene ID (Entrez):
973
Immunogen:
Synthetic peptide corresponding to 202-216 amino acid sequence of human mb-1.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen: IFN-alpha D
Dilution: ELISA, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, Functional, Blocking/Neutralizing 6.25 x 10^4 neatralizing units/mg
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: IFL, IFN, IFNA13, IFNAalphaIFN-alpha-1/13, IFN-ALPHA, IFN-alpha 1b, IFN-alphaD, interferon alpha 1b, interferon alpha-1/13, Interferon alpha-D, interferon, alpha 1, LeIF D, MGC138207, MGC138505, MGC138507
Gene Symbols: IFNA1
Isotype: IgG1
Purification Method: Protein G purified
Test Specificity: NB100-64387 recognizes most isoforms of natural and recombinant murine alpha interferon. It does not cross-react with murine beta or gamma interferon, nor with human alpha interferon.
Clone: F18
Applications: ELISA, Immunohistochemistry, Immunohistochemistry (Frozen), Functional Assay, Blocking Assay
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Cancer, Immunology, Innate Immunity
Gene Accession No.: P01572
Gene ID (Entrez): 3439
Immunogen: Made to Mouse IFN ALPHA
Primary or Secondary: Primary
Content And Storage: Store at -20C. Avoid freeze-thaw cycles.
Antigen:
IFN-alpha D
Dilution:
ELISA, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, Functional, Blocking/Neutralizing 6.25 x 10^4 neatralizing units/mg
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
IFL, IFN, IFNA13, IFNAalphaIFN-alpha-1/13, IFN-ALPHA, IFN-alpha 1b, IFN-alphaD, interferon alpha 1b, interferon alpha-1/13, Interferon alpha-D, interferon, alpha 1, LeIF D, MGC138207, MGC138505, MGC138507
Gene Symbols:
IFNA1
Isotype:
IgG1
Purification Method:
Protein G purified
Test Specificity:
NB100-64387 recognizes most isoforms of natural and recombinant murine alpha interferon. It does not cross-react with murine beta or gamma interferon, nor with human alpha interferon.
Clone:
F18
Applications:
ELISA, Immunohistochemistry, Immunohistochemistry (Frozen), Functional Assay, Blocking Assay
Conjugate:
Unconjugated
Host Species:
Rat
Research Discipline:
Cancer, Immunology, Innate Immunity
Gene Accession No.:
P01572
Gene ID (Entrez):
3439
Immunogen:
Made to Mouse IFN ALPHA
Primary or Secondary:
Primary
Content And Storage:
Store at -20C. Avoid freeze-thaw cycles.
Antigen: F-Actin
Dilution: Western Blot 1:100-1:500, Flow Cytometry 1:10, ELISA 1:10, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Rabbit
Gene Alias: actin, cytoplasmic 2, DFNA26, ACT, ACTG, actin, gamma 1, BRWS2, DFNA20
Gene Symbols: ACTG1
Isotype: IgM
Purification Method: IgM purified
Test Specificity: Recognizes human Filamentous actin (F-actin). The binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.
Clone: NH3
Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: __
Gene Accession No.: P63261
Gene ID (Entrez): 71
Immunogen: Human monocytes and U937 cell line
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen:
F-Actin
Dilution:
Western Blot 1:100-1:500, Flow Cytometry 1:10, ELISA 1:10, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Rabbit
Gene Alias:
actin, cytoplasmic 2, DFNA26, ACT, ACTG, actin, gamma 1, BRWS2, DFNA20
Gene Symbols:
ACTG1
Isotype:
IgM
Purification Method:
IgM purified
Test Specificity:
Recognizes human Filamentous actin (F-actin). The binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.
Clone:
NH3
Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
__
Gene Accession No.:
P63261
Gene ID (Entrez):
71
Immunogen:
Human monocytes and U937 cell line
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.