Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin)
Target Species:
Human, Mouse, Rat, Xenopus species
Immunogen:
Alix fusion protein
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Dilution:
Western Blot 1:100-1:2000, Flow Cytometry, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500
Classification:
Monoclonal
Gene Alias:
AIP1DRIP4, ALG-2 interacting protein 1, ALG-2 interacting protein X, ALG-2-interacting protein 1, alinx, ALIX, apoptosis-linked gene 2-interacting protein X, dopamine receptor interacting protein 4, HP95, KIAA1375, MGC17003, PDCD6-interacting protein, programmed cell death 6 interacting protein, programmed cell death 6-interacting protein
Purification Method:
Protein G purified
Test Specificity:
NB100-65678 recognizes apoptotic-linked-gene-product 2 (ALG-2) interacting protein X (ALIX), a conserved adaptor protein which is ubiquitously expressed. Alix was originally reported to play a role in apoptosis but has recently been shown to be involved in other cellular mechanisms including endosomal sorting, endocytosis, viral budding and actin cytoskeleton assembly.