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7106170

GLI-2 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 6 publications

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

GLI-2

Dilution

Western Blot 1:500-1:2000, ELISA 1:10000-1:30000, Immunohistochemistry 1:500-1:2000, Immunohistochemistry-Paraffin 1:10-1:500, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP) 1:10-1:500

Classification

Polyclonal

Host Species

Rabbit

Research Discipline

Cancer, Stem Cell Signaling Pathway

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide

Gene Alias

GLI family zinc finger 2, GLI-Kruppel family member GLI2, glioma-associated oncogene family zinc finger 2, HPE9tax helper protein 1, oncogene GLI2, Tax helper protein, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein, THP, THP1, THP2, zinc finger protein GLI2

Gene Symbols

GLI2

Isotype

IgG

Purification Method

Affinity Purified

Test Specificity

NB600-874 is expected to cross-react with all 4 splice variants of Human Gli as the immunogen sequence is present in all 4. Tested by Western blotting on human lung, gives low background, but no band was detected.

Concentration

1 mg/ml

Applications

Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Flow Cytometry

Conjugate

Unconjugated

Regulatory Status

RUO

Target Species

Human, Mouse, Rat

Gene Accession No.

P10070

Gene ID (Entrez)

2736

Immunogen

This affinity purified GLI-2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-65 of human GLI-2 (isoform a). (Uniprot: P10070)

Primary or Secondary

Primary

Content And Storage

Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen

167.78 kDa

Description

  • GLI-2 Polyclonal specifically detects GLI-2 in Human, Mouse, Rat samples
  • It is validated for Western Blot, Chromatin Immunoprecipitation, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Flow (Intracellular).

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Img

Fischer Scientific

7106170

Rabbit Polyclonal Antibody has been...

Antigen:
GLI-2
Dilution:
Western Blot 1:500-1:2000, ELISA 1:10000-1:30000, Immunohistochemistry 1:500-1:2000, Immunohistochemistry-Paraffin 1:10-1:500, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP) 1:10-1:500
Classification:
Polyclonal
Host Species:
Rabbit
Research Discipline:
Cancer, Stem Cell Signaling Pathway
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
Gene Alias:
GLI family zinc finger 2, GLI-Kruppel family member GLI2, glioma-associated oncogene family zinc finger 2, HPE9tax helper protein 1, oncogene GLI2, Tax helper protein, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein, THP, THP1, THP2, zinc finger protein GLI2
Gene Symbols:
GLI2
Isotype:
IgG
Purification Method:
Affinity Purified
Test Specificity:
NB600-874 is expected to cross-react with all 4 splice variants of Human Gli as the immunogen sequence is present in all 4. Tested by Western blotting on human lung, gives low background, but no band was detected.
Concentration:
1 mg/ml
Applications:
Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Flow Cytometry
Conjugate:
Unconjugated
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat
Gene Accession No.:
P10070
Gene ID (Entrez):
2736
Immunogen:
This affinity purified GLI-2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-65 of human GLI-2 (isoform a). (Uniprot: P10070)
Primary or Secondary:
Primary
Content And Storage:
Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen:
167.78 kDa

Img

Fischer Scientific

7106173

Mouse Monoclonal Antibody has been ...

Antigen:
Nanog
Dilution:
Western Blot 1:500-1:1000, Flow Cytometry, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:100
Classification:
Monoclonal
Host Species:
Mouse
Research Discipline:
Embryonic Stem Cell Markers, Stem Cell Markers
Formulation:
__
Gene Alias:
FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox
Gene Symbols:
NANOG
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
Anti-human Nanog mAb, clone 5A10, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Nanog protein.
Concentration:
1.0 mg/mL
Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Regulatory Status:
RUO
Target Species:
Human, Mouse
Gene Accession No.:
Q9H9S0
Gene ID (Entrez):
79923
Immunogen:
Recombinant human Nanog (1-154aa) purified from E. coli (NP_079141).
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen:
35 kDa

Img

Fischer Scientific

7106175

Mouse Monoclonal Antibody has been ...

Antigen:
Collagen III alpha 1/COL3A1
Dilution:
__
Classification:
Monoclonal
Host Species:
Mouse
Research Discipline:
__
Formulation:
__
Gene Alias:
alpha1 (III) collagen, Collagen 3, collagen alpha-1(III) chain, collagen, fetal, collagen, type III, alpha 1, Collagen-3, EDS4A, Ehlers-Danlos syndrome type IV, autosomal dominant, FLJ34534
Gene Symbols:
COL3A1
Isotype:
IgG1 κ
Purification Method:
Protein A or G purified
Test Specificity:
Highly specific for type III collagen. It has been shown to have no cross reactivity with type I and V collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
Concentration:
1.0 mg/mL
Applications:
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate:
Unconjugated
Regulatory Status:
RUO
Target Species:
Human, Rat, Canine, Kangaroo, Rabbit
Gene Accession No.:
__
Gene ID (Entrez):
1281
Immunogen:
__
Primary or Secondary:
Primary
Content And Storage:
Store at 4C in the dark.
Molecular Weight of Antigen:
__

Img

Fischer Scientific

7106175

Mouse Monoclonal Antibody has been ...

Antigen:
Collagen III alpha 1/COL3A1
Dilution:
__
Classification:
Monoclonal
Host Species:
Mouse
Research Discipline:
__
Formulation:
__
Gene Alias:
alpha1 (III) collagen, Collagen 3, collagen alpha-1(III) chain, collagen, fetal, collagen, type III, alpha 1, Collagen-3, EDS4A, Ehlers-Danlos syndrome type IV, autosomal dominant, FLJ34534
Gene Symbols:
COL3A1
Isotype:
IgG1 κ
Purification Method:
Protein A or G purified
Test Specificity:
Highly specific for type III collagen. It has been shown to have no cross reactivity with type I and V collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
Concentration:
1.0 mg/mL
Applications:
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate:
Unconjugated
Regulatory Status:
RUO
Target Species:
Human, Rat, Canine, Kangaroo, Rabbit
Gene Accession No.:
__
Gene ID (Entrez):
1281
Immunogen:
__
Primary or Secondary:
Primary
Content And Storage:
Store at 4C in the dark.
Molecular Weight of Antigen:
__