CD44 Monoclonal Antibody (IM7), Super Bright™ 645, eBioscience™, Invitrogen™

Description

• Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA)
• CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets
• The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes
• Applications Reported: This 30-F11 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm
• We recommend using a 660/20 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat
• SB-4400) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer for more information
• CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast
• Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues
• CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration
• CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing
• CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential
• CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes
• Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events
• Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined
• Alternative splicing is the basis for the structural and functional diversity of the CD44 protein
• Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus
• CD44 also may be related to tumor metastasis formation.