CD4 Monoclonal Antibody (RPA-T4), Super Bright™ 645, eBioscience™, Invitrogen™

Description

• Description: The UCHT2 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by a majority of thymocytes and mature T cells and a subset of B cells
• Signaling through the CD5 molecule activates T cells and binding of CD5 to its ligand on B cells, CD72, and plays an important role in T-B interaction and proliferation
• The monoclonal antibody UCHT2 recognizes primate CD5
• Applications Reported: This UCHT2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This UCHT2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm
• We recommend using a 660/20 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No
• SB-4400) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer for more information
• The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV
• CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells
• At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues
• CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck
• It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system
• Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].