IgE Monoclonal Antibody (Ige21), Super Bright™ 780, eBioscience™, Invitrogen™

Description

• Description: The 2D1 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA)
• CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets
• The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes
• Applications Reported: This 2D1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 2D1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm
• We recommend using a 780/60 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product No
• 01-2222-42) as compared to single-color stained cells
• In such circumstances, we would recommend setting compensation with cells
• IgE is one of the five classes of immunoglobulins found in mammals
• Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains
• IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells
• Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations
• IgE is also functionally important for immunity against parasites.