VISTA Monoclonal Antibody (B7H5DS8), APC, eBioscience™, Invitrogen™

Description

• Description: This B7H5DS8 monoclonal antibody recognizes human VISTA also known as B7-H5 or PD-H1
• This clone has been tested and shown not to cross-react with human B7-H4, B7-H5 and B7-H7
• This B7H5DS8 antibody will work in flow cytometry only on live and not paraformaldehyde-fixed cells
• Applications Reported: This B7H5DS8 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This B7H5DS8 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene
• Although VISTA bears sequence homology to PD-L1 it has a different expression pattern
• In both mouse and humans, VISTA is expressed predominantly on hematopoietic cells with the highest densities on myeloid cells, and lower levels on T cells
• It is usually absent on B cells
• VISTA expressed by antigen presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy
• The reported ligands of VISTA include CD28H, VSIG-3 and VSIG-8.