CD42b Monoclonal Antibody (HIP1), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• Description: The HIP1 monoclonal antibody reacts with the human CD42b molecule, a 135 kDa transmembrane protein also known as platelet gpIb
• Applications Reported: This HIP1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HIP1 antibody has been pre-diluted and tested by flow cytometric analysis of human platelets
• This may be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
• Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser CD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor
• It interacts with extracellular matrix as well as with adhesion molecules
• CD42b expression and activation seems to be regulated by VEGF and PDGF
• CD42b composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface
• CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin
• The extracellular domain of CD42b by its interactions also contributes to metastasis
• Further, CD42b antigen is expressed on platelets and on megakaryocytes in bone marrow
• Several polymorphisms and mutations have been described in the CD42b, some of which are the cause of Bernard-Soulier syndromes and platelet-type von Willebrand disease.