7161254
CXCL12/SDF-1 alpha Antibody - Azide and BSA Free, Novus Biologicals™
Rabbit Polyclonal Antibody has been used in 4 publications
Manufacturer: Fischer Scientific
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Antigen
CXCL12/SDF-1 alpha
Dilution
Western Blot 0.1-0.2μ/mL, ELISA 0.5-2.0μ/mL, Immunohistochemistry-Paraffin 0.5μ/mL, Functional 2.0-4.0μ/mL
Classification
Polyclonal
Host Species
Rabbit
Formulation
PBS with no preservative
Gene ID (Entrez)
6387
Immunogen
Antisera to human SDF 1 alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Primary or Secondary
Primary
Content And Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Concentration
LYOPH
Applications
Western Blot, ELISA, Immunohistochemistry (Paraffin), Functional Assay
Conjugate
Unconjugated
Target Species
Human, Rat
Gene Alias
CXCL12 chemokine (C-X-C motif) ligand 12, IRH, PBSF, SCYB12, SDF1, SDF1A, SDF1B, TLSF, TPAR1
Gene Symbols
CXCL12
Isotype
IgG
Reconstitution
Reconstitute with 0.1 mL distilled water. Add 0.09% Sodium Azide for long term storage.
Test Specificity
NBP2-29480 recognises human SDF 1 alpha, otherwise known as CXCL12a, a 68 amino acid stromal cell derived CXC chemokine, which arises from alternative splicing of the SDF 1 gene. The two isoforms, SDF 1 alpha and SDF 1 beta, share an identical amino acid sequence, except for an additional four residues in the C terminal region of the beta isoform.
Description
- CXCL12/SDF-1 alpha Polyclonal specifically detects CXCL12/SDF-1 alpha in Human, Rat samples
- It is validated for Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Functional.
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Antigen: CXCL12/SDF-1 alpha
Dilution: Western Blot 0.1-0.2μ/mL, ELISA 0.5-2.0μ/mL, Immunohistochemistry-Paraffin 0.5μ/mL, Functional 2.0-4.0μ/mL
Classification: Polyclonal
Host Species: Rabbit
Formulation: PBS with no preservative
Gene ID (Entrez): 6387
Immunogen: Antisera to human SDF 1 alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Primary or Secondary: Primary
Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Concentration: LYOPH
Applications: Western Blot, ELISA, Immunohistochemistry (Paraffin), Functional Assay
Conjugate: Unconjugated
Target Species: Human, Rat
Gene Alias: CXCL12 chemokine (C-X-C motif) ligand 12, IRH, PBSF, SCYB12, SDF1, SDF1A, SDF1B, TLSF, TPAR1
Gene Symbols: CXCL12
Isotype: IgG
Reconstitution: Reconstitute with 0.1 mL distilled water. Add 0.09% Sodium Azide for long term storage.
Test Specificity: NBP2-29480 recognises human SDF 1 alpha, otherwise known as CXCL12a, a 68 amino acid stromal cell derived CXC chemokine, which arises from alternative splicing of the SDF 1 gene. The two isoforms, SDF 1 alpha and SDF 1 beta, share an identical amino acid sequence, except for an additional four residues in the C terminal region of the beta isoform.
Antigen:
CXCL12/SDF-1 alpha
Dilution:
Western Blot 0.1-0.2μ/mL, ELISA 0.5-2.0μ/mL, Immunohistochemistry-Paraffin 0.5μ/mL, Functional 2.0-4.0μ/mL
Classification:
Polyclonal
Host Species:
Rabbit
Formulation:
PBS with no preservative
Gene ID (Entrez):
6387
Immunogen:
Antisera to human SDF 1 alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Primary or Secondary:
Primary
Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Concentration:
LYOPH
Applications:
Western Blot, ELISA, Immunohistochemistry (Paraffin), Functional Assay
Conjugate:
Unconjugated
Target Species:
Human, Rat
Gene Alias:
CXCL12 chemokine (C-X-C motif) ligand 12, IRH, PBSF, SCYB12, SDF1, SDF1A, SDF1B, TLSF, TPAR1
Gene Symbols:
CXCL12
Isotype:
IgG
Reconstitution:
Reconstitute with 0.1 mL distilled water. Add 0.09% Sodium Azide for long term storage.
Test Specificity:
NBP2-29480 recognises human SDF 1 alpha, otherwise known as CXCL12a, a 68 amino acid stromal cell derived CXC chemokine, which arises from alternative splicing of the SDF 1 gene. The two isoforms, SDF 1 alpha and SDF 1 beta, share an identical amino acid sequence, except for an additional four residues in the C terminal region of the beta isoform.
Antigen: Cytokeratin 3/2p
Dilution: Western Blot 1:500, Immunohistochemistry-Paraffin 1:50, Immunohistochemistry-Frozen 1:50
Classification: Monoclonal
Host Species: Mouse
Formulation: PBS, pH 7.2, 0.5% BSA. with 0.09% sodium azide
Gene ID (Entrez): 3850
Immunogen: Rabbit corneal epithelial keratin
Primary or Secondary: Primary
Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Concentration: __
Applications: Western Blot, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate: Unconjugated
Target Species: Human, Mouse, Bovine, Rabbit
Gene Alias: 65 KDa Cytokeratin, CK3, CK-3, cytokeratin-3, Keratin, keratin 3, Keratin, Type II Cytoskeletal 3, Keratin-3, Type II Cytoskeletal 3, Type-II keratin Kb3
Gene Symbols: KRT3
Isotype: IgG1
Reconstitution: __
Test Specificity: Recognizes the 64kD polypeptide/keratin K3 (cytokeratin 3) of rabbit corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.
Antigen:
Cytokeratin 3/2p
Dilution:
Western Blot 1:500, Immunohistochemistry-Paraffin 1:50, Immunohistochemistry-Frozen 1:50
Classification:
Monoclonal
Host Species:
Mouse
Formulation:
PBS, pH 7.2, 0.5% BSA. with 0.09% sodium azide
Gene ID (Entrez):
3850
Immunogen:
Rabbit corneal epithelial keratin
Primary or Secondary:
Primary
Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Concentration:
__
Applications:
Western Blot, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate:
Unconjugated
Target Species:
Human, Mouse, Bovine, Rabbit
Gene Alias:
65 KDa Cytokeratin, CK3, CK-3, cytokeratin-3, Keratin, keratin 3, Keratin, Type II Cytoskeletal 3, Keratin-3, Type II Cytoskeletal 3, Type-II keratin Kb3
Gene Symbols:
KRT3
Isotype:
IgG1
Reconstitution:
__
Test Specificity:
Recognizes the 64kD polypeptide/keratin K3 (cytokeratin 3) of rabbit corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.
Antigen: DPP3
Dilution: Western Blot 2 μ/mL, Immunohistochemistry 5-25 μ/mL, Immunocytochemistry 8-25 μ/mL
Classification: Monoclonal
Host Species: Rat
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.
Gene ID (Entrez): 10072
Immunogen: E. coli-derived recombinant human DPP3, Ala2-Ala737, Accession No. Q9NY33
Primary or Secondary: Primary
Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
Concentration: __
Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
Conjugate: Unconjugated
Target Species: Human
Gene Alias: Dipeptidyl aminopeptidase III, Dipeptidyl arylamidase III, dipeptidyl peptidase 3, Dipeptidyl peptidase III, dipeptidylpeptidase 3, dipeptidyl-peptidase 3, dipeptidylpeptidase III, DPP III, DPPIII, EC 3.4.14.4, FLJ11387, FLJ22331
Gene Symbols: __
Isotype: IgG2b
Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity: Detects human DPP3 in direct ELISAs.
Antigen:
DPP3
Dilution:
Western Blot 2 μ/mL, Immunohistochemistry 5-25 μ/mL, Immunocytochemistry 8-25 μ/mL
Classification:
Monoclonal
Host Species:
Rat
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.
Gene ID (Entrez):
10072
Immunogen:
E. coli-derived recombinant human DPP3, Ala2-Ala737, Accession No. Q9NY33
Primary or Secondary:
Primary
Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
Concentration:
__
Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry
Conjugate:
Unconjugated
Target Species:
Human
Gene Alias:
Dipeptidyl aminopeptidase III, Dipeptidyl arylamidase III, dipeptidyl peptidase 3, Dipeptidyl peptidase III, dipeptidylpeptidase 3, dipeptidyl-peptidase 3, dipeptidylpeptidase III, DPP III, DPPIII, EC 3.4.14.4, FLJ11387, FLJ22331
Gene Symbols:
__
Isotype:
IgG2b
Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity:
Detects human DPP3 in direct ELISAs.
Antigen: EGR1
Dilution: Immunohistochemistry 5-25 μ/mL
Classification: Monoclonal
Host Species: Rat
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.
Gene ID (Entrez): 1958
Immunogen: E. coli-derived recombinant human EGR1, Gln282-Ser433, Accession No. P18146
Primary or Secondary: Primary
Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
Concentration: __
Applications: Immunohistochemistry
Conjugate: Unconjugated
Target Species: Human
Gene Alias: AT225Transcription factor ETR103, early growth response 1, early growth response protein 1, EGR-1, G0S30, KROX24, KROX-24, Nerve growth factor-induced protein A, NGFIA, NGFI-ATranscription factor Zif268, TIS8, ZIF-268, Zinc finger protein Krox-24, ZNF225, ZNF225Zinc finger protein 225
Gene Symbols: __
Isotype: IgG2a
Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity: Detects human EGR1 in direct ELISAs.
Antigen:
EGR1
Dilution:
Immunohistochemistry 5-25 μ/mL
Classification:
Monoclonal
Host Species:
Rat
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.
Gene ID (Entrez):
1958
Immunogen:
E. coli-derived recombinant human EGR1, Gln282-Ser433, Accession No. P18146
Primary or Secondary:
Primary
Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
Concentration:
__
Applications:
Immunohistochemistry
Conjugate:
Unconjugated
Target Species:
Human
Gene Alias:
AT225Transcription factor ETR103, early growth response 1, early growth response protein 1, EGR-1, G0S30, KROX24, KROX-24, Nerve growth factor-induced protein A, NGFIA, NGFI-ATranscription factor Zif268, TIS8, ZIF-268, Zinc finger protein Krox-24, ZNF225, ZNF225Zinc finger protein 225
Gene Symbols:
__
Isotype:
IgG2a
Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity:
Detects human EGR1 in direct ELISAs.