CD38 Mouse anti-Human, Brilliant Ultraviolet 737, Clone: HB7, BD Horizon™

Description

• The HB7 monoclonal antibody specifically binds to human CD38
• CD38 is a type II transmembrane glycoprotein of 45 kDa with a protein core of 35 kDa
• The CD38 antigen is expressed on essentially all pre-B lymphocytes, plasma cells, and thymocytes
• It is also present on activated T lymphocytes, natural killer (NK) lymphocytes, myeloblasts, and erythroblasts
• The antigen is expressed during the early stages of T- and B-lymphocyte differentiation, is lost during the intermediate stages of maturation, and then reappears during the final stages of maturation
• The CD38 antigen is expressed on 90% of CD34+ cells, and is not expressed on pluripotent stem cells
• Coexpression of CD38 antigen on CD34+ cells indicates lineage commitment of those cells
• CD38 is a counter-receptor of CD31
• It is also expressed in T- and B-acute lymphoblastic leukemia (ALL), Burkitt's lymphoma, multiple myeloma, acute myeloid leukemia (AML), and chronic lymphocytic leukemia (CLL)
• The antibody was conjugated to BD Horizon BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm
• BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter
• Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter).