7161706
CD3 Mouse anti-Human, Brilliant Ultraviolet 661, Clone: UCHT1, BD Horizon™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD3
Applications
Flow Cytometry
Conjugate
Brilliant Ultraviolet 661
Regulatory Status
RUO
Formulation
Aqueous buffered solution containing ≤0.09% sodium azide.
Isotype
IgG1 κ
Purification Method
Affinity Purified
Clone
UCHT1
Classification
Monoclonal
Host Species
Mouse
Target Species
Human
Immunogen
Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Description
- The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex
- CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes
- Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody
- CD3 plays a central role in signal transduction during antigen recognition
- The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε
- The antibody was conjugated to BD Horizon BUV661 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
- This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 661-nm
- BD Horizon Brilliant BUV661 can be excited by the ultraviolet laser (355 nm) and detected with a 670/25 filter and a 630nm LP
- Due to cross laser excitation of this dye, there may be significant spillover into channels detecting APC-like emissions (eg, 670/30-nm filter)
- For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment
- Fluorescent dye interactions may cause staining artifacts which may affect data interpretation
- The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions
- More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Catalog number 563794)
- Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV661 reagents
- Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended
- Different BUV661 reagents (eg, CD4 vs
- CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone-specific compensation controls when using these reagents.
Compare Similar Items
Show Difference
Antigen: CD3
Applications: Flow Cytometry
Conjugate: Brilliant Ultraviolet 661
Regulatory Status: RUO
Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
Isotype: IgG1 κ
Purification Method: Affinity Purified
Clone: UCHT1
Classification: Monoclonal
Host Species: Mouse
Target Species: Human
Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen:
CD3
Applications:
Flow Cytometry
Conjugate:
Brilliant Ultraviolet 661
Regulatory Status:
RUO
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Isotype:
IgG1 κ
Purification Method:
Affinity Purified
Clone:
UCHT1
Classification:
Monoclonal
Host Species:
Mouse
Target Species:
Human
Immunogen:
Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen: CD3
Applications: Flow Cytometry
Conjugate: Brilliant Ultraviolet 737
Regulatory Status: RUO
Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
Isotype: IgG1 κ
Purification Method: Affinity Purified
Clone: UCHT1
Classification: Monoclonal
Host Species: Mouse
Target Species: Human
Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen:
CD3
Applications:
Flow Cytometry
Conjugate:
Brilliant Ultraviolet 737
Regulatory Status:
RUO
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Isotype:
IgG1 κ
Purification Method:
Affinity Purified
Clone:
UCHT1
Classification:
Monoclonal
Host Species:
Mouse
Target Species:
Human
Immunogen:
Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen: CD38
Applications: Flow Cytometry
Conjugate: Brilliant Ultraviolet 496
Regulatory Status: RUO
Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
Isotype: IgG1 κ
Purification Method: Affinity Purified
Clone: HIT2
Classification: Monoclonal
Host Species: Mouse
Target Species: Human
Immunogen: __
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen:
CD38
Applications:
Flow Cytometry
Conjugate:
Brilliant Ultraviolet 496
Regulatory Status:
RUO
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Isotype:
IgG1 κ
Purification Method:
Affinity Purified
Clone:
HIT2
Classification:
Monoclonal
Host Species:
Mouse
Target Species:
Human
Immunogen:
__
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen: CD11c
Applications: Flow Cytometry
Conjugate: Brilliant Ultraviolet 661
Regulatory Status: RUO
Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
Isotype: IgG1 κ
Purification Method: Affinity Purified
Clone: B-ly6
Classification: Monoclonal
Host Species: Mouse
Target Species: Human
Immunogen: Spleen Cells from Human with Hairy Cell Leukemia
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen:
CD11c
Applications:
Flow Cytometry
Conjugate:
Brilliant Ultraviolet 661
Regulatory Status:
RUO
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Isotype:
IgG1 κ
Purification Method:
Affinity Purified
Clone:
B-ly6
Classification:
Monoclonal
Host Species:
Mouse
Target Species:
Human
Immunogen:
Spleen Cells from Human with Hairy Cell Leukemia
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.