7161721

CD14 Mouse anti-Baboon, Cynomolgus, Human, Rhesus, Brilliant Ultraviolet 737, Clone: M5E2, BD Horizon™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD14

Applications

Flow Cytometry

Conjugate

Brilliant Ultraviolet 737

Regulatory Status

RUO

Formulation

Aqueous buffered solution containing ≤0.09% sodium azide.

Isotype

IgG2a κ

Purification Method

Affinity Purified

Clone

M5E2

Classification

Monoclonal

Host Species

Mouse

Target Species

Baboon, Cynomolgus, Human, Rhesus Monkey

Immunogen

Human CD14 Protein

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Description

  • The M5E2 monoclonal antibody specifically binds to CD14, a 53–55 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein expressed at high levels on monocytes
  • Additionally, the anti-CD14 antibody reacts with interfollicular macrophages, reticular dendritic cells, and some Langerhans cells
  • CD14 has been identified as a high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein, LPB
  • The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
  • This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm
  • BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter
  • Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor™ 700-like dyes (e.g., 712/20-nm filter).

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Antigen:
CD14

Applications:
Flow Cytometry

Conjugate:
Brilliant Ultraviolet 737

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Isotype:
IgG2a κ

Purification Method:
Affinity Purified

Clone:
M5E2

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Baboon, Cynomolgus, Human, Rhesus Monkey

Immunogen:
Human CD14 Protein

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Aqueous buffered solution containing ≤0.09% sodium azide.

Isotype:
__

Purification Method:
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Clone:
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Host Species:
Mouse

Target Species:
Baboon, Human, Rhesus Monkey

Immunogen:
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Primary or Secondary:
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Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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CD16

Applications:
Flow Cytometry

Conjugate:
Brilliant Ultraviolet 737

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Aqueous buffered solution containing ≤0.09% sodium azide.

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Clone:
3G8

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Baboon, Human, Rhesus Monkey

Immunogen:
Human polymorphonuclear leukocytes

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.