7162225
Lambda light chain Monoclonal Antibody (1-155-2), Super Bright™ 436, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Lambda light chain
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with BSA and 0.09% sodium azide, pH 7.2
Gene Alias
Bence Jones Protein, BJP, Clambda1, Ig lambda chain V-I region BL2, IgL, Igl@, IGLC, IGLC 1/2/3, IGLC1, Igl-C1, IGLL5, IGLV, IGLV@, immmunoglobulin lambda light chain variable region, Immunoglobulin L, immunoglobulin lambda chain complex, immunoglobulin lambda constant 1, immunoglobulin lambda constant 7, immunoglobulin lambda light chain, immunoglobulin lambda light chain constant region, immunoglobulin lambda light chain V-J region, immunoglobulin lambda variable cluster, Lambda LC, lambda-immunoglobulin, LOC100060608, Mcg Marker, Paraprotein
Gene Symbols
IGL, IGLC1, IGLV, IGLV1-51
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Clone
1-155-2
Applications
Flow Cytometry
Conjugate
Super Bright 436
Host Species
Mouse
Target Species
Human
Gene Accession No.
P01701, P0CG04
Gene ID (Entrez)
28820, 3535, 3537, 3546
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Description
- Description: The monoclonal antibody 1-155-2 recognizes the lambda light chain of human immunoglobulin
- Approximately 22-kDa, the Ig lambda chain is comprised of one variable and one constant region
- Two lambda chains together with the heavy chain comprise the whole Ig structure
- Expression of Ig lambda is typically found in less than half of B lymphocytes, and has been associated with neoplastic lymphomas
- Applications Reported: This 1-155-2 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This 1-155-2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- This may be used at 5 μL (0.5 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
- We recommend using a 450/50 bandpass filter, or equivalent
- Please make sure that your instrument is capable of detecting this fluorochrome
- When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No
- SB-4401) to minimize any non-specific polymer interactions
- Please refer to the datasheet for Super Bright Staining Buffer for more information
- Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five types) and two light chains (kappa, lambda; both having a molecular weight of 22.5 kDa)
- Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region
- The ratio of kappa to lambda is 70:30
- Antibodies to the kappa light chain are reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas
- Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
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Antigen: Lambda light chain
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with BSA and 0.09% sodium azide, pH 7.2
Gene Alias: Bence Jones Protein, BJP, Clambda1, Ig lambda chain V-I region BL2, IgL, Igl@, IGLC, IGLC 1/2/3, IGLC1, Igl-C1, IGLL5, IGLV, IGLV@, immmunoglobulin lambda light chain variable region, Immunoglobulin L, immunoglobulin lambda chain complex, immunoglobulin lambda constant 1, immunoglobulin lambda constant 7, immunoglobulin lambda light chain, immunoglobulin lambda light chain constant region, immunoglobulin lambda light chain V-J region, immunoglobulin lambda variable cluster, Lambda LC, lambda-immunoglobulin, LOC100060608, Mcg Marker, Paraprotein
Gene Symbols: IGL, IGLC1, IGLV, IGLV1-51
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: 1-155-2
Applications: Flow Cytometry
Conjugate: Super Bright 436
Host Species: Mouse
Target Species: Human
Gene Accession No.: P01701, P0CG04
Gene ID (Entrez): 28820, 3535, 3537, 3546
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
Lambda light chain
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with BSA and 0.09% sodium azide, pH 7.2
Gene Alias:
Bence Jones Protein, BJP, Clambda1, Ig lambda chain V-I region BL2, IgL, Igl@, IGLC, IGLC 1/2/3, IGLC1, Igl-C1, IGLL5, IGLV, IGLV@, immmunoglobulin lambda light chain variable region, Immunoglobulin L, immunoglobulin lambda chain complex, immunoglobulin lambda constant 1, immunoglobulin lambda constant 7, immunoglobulin lambda light chain, immunoglobulin lambda light chain constant region, immunoglobulin lambda light chain V-J region, immunoglobulin lambda variable cluster, Lambda LC, lambda-immunoglobulin, LOC100060608, Mcg Marker, Paraprotein
Gene Symbols:
IGL, IGLC1, IGLV, IGLV1-51
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
1-155-2
Applications:
Flow Cytometry
Conjugate:
Super Bright 436
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P01701, P0CG04
Gene ID (Entrez):
28820, 3535, 3537, 3546
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: ROR gamma (t)
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide, pH 7.2
Gene Alias: EGK_01272, IMD42, MGC129539, NR1F3, nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, nuclear receptor subfamily 1 group F member 3, RAR related orphan receptor C, RAR-related orphan nuclear receptor variant 2, RAR-related orphan receptor C, RAR-related orphan receptor C, isoform a, RAR-related orphan receptor gamma, retinoic acid-binding receptor gamma, retinoid-related orphan receptor gamma, retinoid-related orphan receptor-gamma, ROR Gamma, ROR gamma t, Rorc, Rorg, RORgamma, RORgt, RP11-98D18.11-001, RZRG, RZR-GAMMA, Thor, Thymus orphan receptor, TOR
Gene Symbols: Rorc
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: AFKJS-9
Applications: Flow Cytometry
Conjugate: PerCP-eFluor 710
Host Species: Rat
Target Species: Human, Mouse, Rhesus Monkey
Gene Accession No.: P51449, P51450
Gene ID (Entrez): 19885, 6097, 717052
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
ROR gamma (t)
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide, pH 7.2
Gene Alias:
EGK_01272, IMD42, MGC129539, NR1F3, nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, nuclear receptor subfamily 1 group F member 3, RAR related orphan receptor C, RAR-related orphan nuclear receptor variant 2, RAR-related orphan receptor C, RAR-related orphan receptor C, isoform a, RAR-related orphan receptor gamma, retinoic acid-binding receptor gamma, retinoid-related orphan receptor gamma, retinoid-related orphan receptor-gamma, ROR Gamma, ROR gamma t, Rorc, Rorg, RORgamma, RORgt, RP11-98D18.11-001, RZRG, RZR-GAMMA, Thor, Thymus orphan receptor, TOR
Gene Symbols:
Rorc
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
AFKJS-9
Applications:
Flow Cytometry
Conjugate:
PerCP-eFluor 710
Host Species:
Rat
Target Species:
Human, Mouse, Rhesus Monkey
Gene Accession No.:
P51449, P51450
Gene ID (Entrez):
19885, 6097, 717052
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: TCR beta
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with BSA and 0.09% sodium azide, pH 7.2
Gene Alias: bTCR, FLJ22602, MGC117436, MGC22624, MGC23964, MGC71411, T cell receptor beta chain, t-cell antigen receptor, T-cell receptor, TCR beta, Tcra, Tcrb, TCRbeta, TCRD, Tib, TRA
Gene Symbols: Tcrb
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: H57-597
Applications: Flow Cytometry
Conjugate: Super Bright 780
Host Species: Armenian Hamster
Target Species: Mouse
Gene Accession No.: __
Gene ID (Entrez): 21577
Isotype: IgG
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
TCR beta
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with BSA and 0.09% sodium azide, pH 7.2
Gene Alias:
bTCR, FLJ22602, MGC117436, MGC22624, MGC23964, MGC71411, T cell receptor beta chain, t-cell antigen receptor, T-cell receptor, TCR beta, Tcra, Tcrb, TCRbeta, TCRD, Tib, TRA
Gene Symbols:
Tcrb
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
H57-597
Applications:
Flow Cytometry
Conjugate:
Super Bright 780
Host Species:
Armenian Hamster
Target Species:
Mouse
Gene Accession No.:
__
Gene ID (Entrez):
21577
Isotype:
IgG
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: __
Concentration: __
Classification: __
Form: __
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