CD138 (Syndecan-1) Monoclonal Antibody (MI15), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: This MI15 monoclonal antibody reacts with human CD138, also known as syndecan-1
• CD138 is a transmembrane protein containing chondroitin sulfate and heparin sulfate moieties responsible for binding to the extracellular matrix components
• CD138 is not expressed by mature B cells but is present on pre-B cells and the finally differentiated plasma cells
• Applications Reported: This MI15 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This MI15 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells
• This may be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
• We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
• Please make sure that your instrument is capable of detecting this fluorochrome
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (Product No
• CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes
• CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury
• It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells
• Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics
• Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.