7182707

CD28, Rabbit anti-Rat, Clone: JJ316, Novus Biologicals™

Rabbit Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

CD28

Concentration

1 mg/mL

Applications

Flow Cytometry, Immunohistochemistry, Immunofluorescence, Functional Assay, Blocking Assay

Conjugate

Unconjugated

Host Species

Rabbit

Research Discipline

Adaptive Immunity, Apoptosis, Immunology

Formulation

PBS

Gene ID (Entrez)

940

Immunogen

Made by immunizing BALB/c mice with rat CD28 cDNA-transfected A20/J cells.

Primary or Secondary

Primary

Content And Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Clone

JJ316

Dilution

Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Functional, Block/Neutralize

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Rat

Gene Alias

CD28 antigen, CD28 antigen (Tp44), CD28 molecule, MGC138290, T-cell-specific surface glycoprotein CD28, Tp44

Gene Symbols

CD28

Isotype

IgG κ

Purification Method

Protein A purified

Test Specificity

Recognises rat CD28 and can trigger T-cell proliferation in vitro and in vivo.

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Description

  • CD28 Monoclonal specifically detects CD28 in Rat samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Functional, Block/Neutralize.

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Fischer Scientific

7182707

Rabbit Monoclonal Antibody...


Antigen:
CD28

Concentration:
1 mg/mL

Applications:
Flow Cytometry, Immunohistochemistry, Immunofluorescence, Functional Assay, Blocking Assay

Conjugate:
Unconjugated

Host Species:
Rabbit

Research Discipline:
Adaptive Immunity, Apoptosis, Immunology

Formulation:
PBS

Gene ID (Entrez):
940

Immunogen:
Made by immunizing BALB/c mice with rat CD28 cDNA-transfected A20/J cells.

Primary or Secondary:
Primary

Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Clone:
JJ316

Dilution:
Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Functional, Block/Neutralize

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Rat

Gene Alias:
CD28 antigen, CD28 antigen (Tp44), CD28 molecule, MGC138290, T-cell-specific surface glycoprotein CD28, Tp44

Gene Symbols:
CD28

Isotype:
IgG κ

Purification Method:
Protein A purified

Test Specificity:
Recognises rat CD28 and can trigger T-cell proliferation in vitro and in vivo.

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7182708

Rabbit Monoclonal Antibody...


Antigen:
Gr-1/Ly-6G

Concentration:
1 mg/mL

Applications:
Flow Cytometry

Conjugate:
Unconjugated

Host Species:
Rabbit

Research Discipline:
Innate Immunity

Formulation:
PBS

Gene ID (Entrez):
58530

Immunogen:
LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells

Primary or Secondary:
Primary

Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Clone:
1A8

Dilution:
Flow Cytometry

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Mouse

Gene Alias:
C6orf23, G6D, LY6-D, LY6G6D, lymphocyte antigen 6 family member G6D, MEGT1, NG25

Gene Symbols:
LY6G6D

Isotype:
IgG κ

Purification Method:
Protein A purified

Test Specificity:
Recognises the murine GPI-anchored protein Ly6G, a marker of granulocyte differentiation, maturation and, together with Ly6C, a component of the Granulocyte Receptor-1 Antigen (Gr-1). Unlike another commonly used clone, RB6-8C5, 1A8 does not bind Ly6C.

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7182709

Rabbit Monoclonal Antibody...


Antigen:
CD28

Concentration:
1 mg/mL

Applications:
Flow Cytometry, Immunohistochemistry, Functional Assay, Blocking Assay

Conjugate:
Unconjugated

Host Species:
Rabbit

Research Discipline:
Adaptive Immunity, Apoptosis, Immunology

Formulation:
PBS

Gene ID (Entrez):
940

Immunogen:
The original D665 antibody was generated by immunizing mice alternately with A20 cells expressing mCD28 i.p. and with recombinant mCD28Ig s.c.

Primary or Secondary:
Primary

Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Clone:
D665

Dilution:
Flow Cytometry, Immunohistochemistry, Functional, Surface Plasmon Resonance, Block/Neutralize

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Mouse

Gene Alias:
CD28 antigen, CD28 antigen (Tp44), CD28 molecule, MGC138290, T-cell-specific surface glycoprotein CD28, Tp44

Gene Symbols:
CD28

Isotype:
IgG κ

Purification Method:
Protein A purified

Test Specificity:
This antibody binds to the laterally exposed C'D loop of CD28 and induces T cell proliferation without TCR ligation.

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7182709

Rabbit Monoclonal Antibody...


Antigen:
CD28

Concentration:
1 mg/mL

Applications:
Flow Cytometry, Immunohistochemistry, Functional Assay, Blocking Assay

Conjugate:
Unconjugated

Host Species:
Rabbit

Research Discipline:
Adaptive Immunity, Apoptosis, Immunology

Formulation:
PBS

Gene ID (Entrez):
940

Immunogen:
The original D665 antibody was generated by immunizing mice alternately with A20 cells expressing mCD28 i.p. and with recombinant mCD28Ig s.c.

Primary or Secondary:
Primary

Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Clone:
D665

Dilution:
Flow Cytometry, Immunohistochemistry, Functional, Surface Plasmon Resonance, Block/Neutralize

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Mouse

Gene Alias:
CD28 antigen, CD28 antigen (Tp44), CD28 molecule, MGC138290, T-cell-specific surface glycoprotein CD28, Tp44

Gene Symbols:
CD28

Isotype:
IgG κ

Purification Method:
Protein A purified

Test Specificity:
This antibody binds to the laterally exposed C'D loop of CD28 and induces T cell proliferation without TCR ligation.