7190542

DbpB Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

DbpB

Applications

Immunoprecipitation, Western Blot

Conjugate

Unconjugated

Host Species

Rabbit

Target Species

Bacteria

Immunogen

MBP-fusion protein corresponding to Borrelia burgdorferi Dbp-B protein.

Content And Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Concentration

1 mg/mL

Classification

Polyclonal

Form

Lyophilized

Regulatory Status

RUO

Formulation

0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2

Primary or Secondary

Primary

Product Type

Antibody

Description

  • Store vial at 4°C prior to restoration
  • For extended storage aliquot contents and freeze at -20°C or below
  • Avoid cycles of freezing and thawing
  • Centrifuge product if not completely clear after standing at room temperature
  • This product is stable for several weeks at 4°C as an undiluted liquid
  • Dilute only prior to immediate use
  • This antibody is specific for Borrelia burgdorferi DbpB protein
  • A BLAST analysis was used to suggest cross-reactivity with DbpB from B
  • burgdorferi and B
  • garinii sources based on 100% homology with the immunizing sequence
  • Cross-reactivity with DbpB from other sources has not been determined
  • Decorin-binding protein B, or DbpB, binds to decorin, which may mediate the adherence of B.burgdorferi to collagen fibers in skin and other tissues
  • Spirochetal surface adhesions mediate attachment to decorin, a major component of the host extracellular matrix enabling bacteria to colonize in mammalian tissues
  • The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host
  • This transition may be facilitated by changes in expression of some B
  • burgdorferi genes
  • It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host
  • Borrelia burgdorferi can colonize multiple tissues, and is capable of attachment to diverse cell types
  • The expression of decorin-binding protein (Dbp) A and/or DbpB, two B
  • burgdorferi surface proteins that bind GAGs, is sufficient to convert a high-passage nonadherent B
  • burgdorferi strain into one that efficiently binds 293 epithelial cells.

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Antigen:
DbpB

Applications:
Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Host Species:
Rabbit

Target Species:
Bacteria

Immunogen:
MBP-fusion protein corresponding to Borrelia burgdorferi Dbp-B protein.

Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Concentration:
1 mg/mL

Classification:
Polyclonal

Form:
Lyophilized

Regulatory Status:
RUO

Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2

Primary or Secondary:
Primary

Product Type:
Antibody

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Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Conjugate:
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Host Species:
Goat

Target Species:
Human

Immunogen:
Anti-alpha 1 Anti-Trypsin Antibody was produced by repeated immunizations with native alpha1-Anti-Trypsin protein from human plasma.

Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Concentration:
1 mg/mL

Classification:
Polyclonal

Form:
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Applications:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot

Conjugate:
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Host Species:
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Target Species:
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Immunogen:
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Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles

Concentration:
0.65 mg/mL

Classification:
Polyclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2

Primary or Secondary:
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Applications:
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Conjugate:
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Host Species:
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Target Species:
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Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Concentration:
1 mg/mL

Classification:
Polyclonal

Form:
Lyophilized

Regulatory Status:
RUO

Formulation:
0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2

Primary or Secondary:
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Product Type:
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