Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Anti-SMAD2 pS465 pS467 antibody is directed against the phosphorylated form of human Smad2 protein at the pS465 and pS467 residues
• Reactivity occurs against human SMAD2
• Reactivity with non-phosphorylated human Smad2 is minimal by ELISA and western blot
• A BLAST analysis was used to suggest cross-reactivity with Smad2 protein from human, mouse, rat, orangutan, and bovine based on 100% homology with the immunizing sequence
• Reactivity against homologues from other sources is not known
• SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation
• SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling
• Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin
• In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors
• The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4
• The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors
• SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin
• Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.