7190574
DYKDDDDK Monoclonal Antibody (29E4.G7), Rockland™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
DYKDDDDK
Concentration
1 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Gene Alias
DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Isotype
IgG2a κ
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Clone
29E4.G7
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Formulation
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAGTM epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Primary or Secondary
Primary
Product Type
Antibody
Description
- Store vial at -20°C prior to opening
- Aliquot contents and freeze at -20°C or below for extended storage
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- The purified antibody is directed against the FLAGTM motif and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins
- This monoclonal anti-FLAGTM tag antibody detects over-expressed proteins containing the FLAGTM epitope tag
- In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins
- DYKDDDDK Tag (FLAG™ tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology
- The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended
- A FLAG tag can be used in many different assays that require recognition by an antibody
- If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence
- Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis
- Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein
- The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function
- The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins
- FLAG is a trademark of Sigma-Aldrich Biotechnology LP
Compare Similar Items
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Antigen: DYKDDDDK
Concentration: 1 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Gene Alias: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Isotype: IgG2a κ
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Clone: 29E4.G7
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAGTM epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Primary or Secondary: Primary
Product Type: Antibody
Antigen:
DYKDDDDK
Concentration:
1 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Gene Alias:
DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Isotype:
IgG2a κ
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Clone:
29E4.G7
Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen:
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAGTM epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Primary or Secondary:
Primary
Product Type:
Antibody
Antigen: E.coli Combined HCP
Concentration: 0.82 mg/mL
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Gene Alias: __
Isotype: __
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Clone: __
Applications: ELISA, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen: Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.Coli (BL21).
Primary or Secondary: Primary
Product Type: Antibody
Antigen:
E.coli Combined HCP
Concentration:
0.82 mg/mL
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Gene Alias:
__
Isotype:
__
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Clone:
__
Applications:
ELISA, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen:
Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.Coli (BL21).
Primary or Secondary:
Primary
Product Type:
Antibody
Antigen: Pax7
Concentration: 1 mg/mL
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Gene Alias: FLJ37460; HUP1; paired box 7; paired box gene 7; paired box homeotic gene 7; Paired box protein Pax-7; paired box transcription factor PAX7; paired domain gene 7; Pax7; Pax-7; PAX7 transcriptional factor; PAX7A; PAX7B; RGD1564360; RMS2
Isotype: __
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Clone: __
Applications: ELISA, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen: __
Primary or Secondary: Primary
Product Type: Antibody
Antigen:
Pax7
Concentration:
1 mg/mL
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Gene Alias:
FLJ37460; HUP1; paired box 7; paired box gene 7; paired box homeotic gene 7; Paired box protein Pax-7; paired box transcription factor PAX7; paired domain gene 7; Pax7; Pax-7; PAX7 transcriptional factor; PAX7A; PAX7B; RGD1564360; RMS2
Isotype:
__
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Clone:
__
Applications:
ELISA, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen:
__
Primary or Secondary:
Primary
Product Type:
Antibody
Antigen: Pax7
Concentration: 1 mg/mL
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Gene Alias: FLJ37460; HUP1; paired box 7; paired box gene 7; paired box homeotic gene 7; Paired box protein Pax-7; paired box transcription factor PAX7; paired domain gene 7; Pax7; Pax-7; PAX7 transcriptional factor; PAX7A; PAX7B; RGD1564360; RMS2
Isotype: __
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Clone: __
Applications: ELISA, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen: __
Primary or Secondary: Primary
Product Type: Antibody
Antigen:
Pax7
Concentration:
1 mg/mL
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Gene Alias:
FLJ37460; HUP1; paired box 7; paired box gene 7; paired box homeotic gene 7; Paired box protein Pax-7; paired box transcription factor PAX7; paired domain gene 7; Pax7; Pax-7; PAX7 transcriptional factor; PAX7A; PAX7B; RGD1564360; RMS2
Isotype:
__
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Clone:
__
Applications:
ELISA, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen:
__
Primary or Secondary:
Primary
Product Type:
Antibody