Phospho-RON (Tyr1360) Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum
• This antibody is specific for human RON protein phosphorylated at Y1360
• Cross-reactivity against RON from other species may occur but has not yet been tested
• Macrophage-stimulatin 1 receptor (MST1R/Ron), a HGF Receptor/MET-type protein kinase, mediates the biological activities of macrophage-stimulating protein (MSP), a multifunctional cytokine that regulates cell adhesion, motility, growth, and survival
• The protein is a membrane-spanning, disulfide-linked heterodimer, which results from cleavage of a glycosylated precursor into 35-kD (alpha) and 150-kD (beta) subunits
• Ligand binding results in tyrosine phosphorylation of the beta chain
• In knockout studies, MST1R/RON (-/-) mice failed to survive past the periimplantation period
• The MST1R/RON gene has been mapped to 3p21, a region of frequent deletion or mutation in small cell lung and renal carcinoma, and has been implicated in the progression of several epithelial cancers
• MST1R/Ron expression has been documented in many normal human tissues
• ESTs have been isolated from several tissue libraries, including normal colon, mouth, prostate, and testis and cancerous colon, prostate, stomach, and uteru.