NFkB p50 Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Anti-Human NFkB p50 (NFKB1) may react non-specifically with other proteins
• Control peptide (code #100-4164p) will compete only with the specific reaction of antiserum with Human NFkB p50 (NFKB1)
• NFkB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products
• NFkB is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation
• NFKB1 (p50) is a 50KDa functional sub-unit of NF-kB and a member of Rel protein family which is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains
• NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NFkB factor
• Further, NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-binding
• The NFkB complex is expressed in most cell types, and is primarily found in the cytoplasm in an inactive state in association with I-kappa-B
• Phosphorylation of I-kappa-B releases the complex, allowing translocation to the nucleus and modification of gene expression
• Phosphorylation of the p50 subunit at the Ser-337 residue has been shown to significantly increase DNA binding efficiency of the NFkB protein complex
• The pathological role of NFkB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.