Sprouty-2 Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Sprouty-2 antibody reacts with human Sprouty 2 protein
• A BLAST analysis was used to suggest reactivity with Sprouty 2 from human, monkey, and orangutan based on a 100% homology with the immunizing sequence
• Cross-reactivity with Sprouty2 from other sources has not been determined
• This gene encodes a protein belonging to the sprouty family
• The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles
• In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two
• This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling
• The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling
• This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein.