Maltose Binding Protein (MBP) Epitope Tag Polyclonal Antibody, Biotin, Rockland™

Description

• Store vial at 4°C prior to restoration
• For extended storage aliquot contents and freeze at -20°C or below
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This IgG purified antibody is directed against MBP and is useful in determining its presence in various assays
• This polyclonal anti-MBP tag antibody detects over-expressed proteins containing the MBP epitope tag
• To date this antibody has reacted with all MBP tagged proteins so far tested
• In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins
• The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system
• However, MBP-related transcripts are also present in the bone marrow and the immune system
• These mRNAs arise from the long MBP gene (otherwise called 'Golli-MBP') that contains 3 additional exons located upstream of the classic MBP exons
• Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products
• The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons
• They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence
• The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins
• This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.