DbpA Polyclonal Antibody, Rockland™

Description

• Store vial at 4°C prior to restoration
• For extended storage aliquot contents and freeze at -20°C or below
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This antibody is specific for Borrelia burgdorferi DbpA protein
• A BLAST analysis was used to suggest cross-reactivity with DbpA from Borrelia burgdorferi sources based on 100% homology with the immunizing sequence
• Partial reactivity is expected against Borrelia garinii sources based on 60-80% homology
• Cross-reactivity with DbpA from other sources has not been determined
• DbpA, or Decorin Binding Protein A, is from the spirochete Borrelia burgdorferi, which is carried by Ixodes ticks
• DbpA from other microbial organisms such as E
• coli (ATP-dependent RNA helicase DbpA) are significantly different
• The spirochete migrates from the tick midgut during tick feeding to tick salivary glands and are thus transmitted to the mammal host
• This transition may be facilitated by changes in expression of some B
• burgdorferi genes
• Spirochetal surface adhesions mediate attachment to decorin, a major component of the host extracellular matrix, enabling bacteria to colonize in mammalian tissues
• It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host.