Peroxidase Polyclonal Antibody, Rockland™

Description

• Store vial at 4°C prior to restoration
• For extended storage aliquot contents and freeze at -20°C or below
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Anti-PEROXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above
• Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Peroxidase [Horseradish]
• Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined
• Chemiluminescent detection systems have emerged as the best all-around method for detection of Western blots
• They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates
• The speed and sensitivity of these methods are unequalled by traditional alternatives
• Because results are generated on film, it is possible to record and store data permanently, and blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies
• HRP (Horseradish Peroxidase) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal
• HRP-antibody conjugates have a very high turnover rate, giving good sensitivity with short reaction times.