7192863
CD200R3 Monoclonal Antibody (Ba13), PE-Cyanine7, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD200R3
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
4733401I18Rik; 4833409J19Rik; AI505817; BB106877; CD200 cell surface glycoprotein receptor 3; CD200 cell surface glycoprotein receptor-like 3; CD200 cell surface glycoprotein receptor-like b; CD200 receptor 3; CD200 receptor-like 3; Cd200r3; CD200RLb; cell surface glycoprotein CD200 receptor 3; Cell surface glycoprotein OX2 receptor 3; mCD200RLb
Gene Symbols
CD200R3
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
Clone
Ba13
Applications
Flow Cytometry
Conjugate
PE-Cyanine7
Host Species
Rat
Target Species
Mouse
Gene Accession No.
Q5UKY4
Gene ID (Entrez)
74603
Immunogen
Mouse primary basophils
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
CD200R3
Description
- Description: This Ba13 monoclonal antibody recognizes mouse CD200R3, a surface receptor expressed by basophils and mast cells
- Applications Reported: This Ba13 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This Ba13 antibody has been tested by flow cytometric analysis of the MC/9 cell line
- This may be used at less than or equal to 0.25 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
- Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
- Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser CD200R3 is a dimeric cell surface receptor also known as CD200RLb
- CD200R3 contains two Ig-like extracellular domains and as such is a member of the immunoglobulin superfamily of receptors
- The intracellular region of CD200R3 associates with the adaptor molecule DAP12, and possibly also DAP10, CD3 zeta and FcR gamma-chain, indicating that it is capable of transducing stimulatory signals
- In this respect CD200R3 bears similarity to activatory receptors CD200R2 and CD200R4
- In contrast, an inhibitory receptor CD200R (also known as CD200R1) relies on other adaptors
- CD200R3 is primarily expressed by basophils and mast cells, cells known to be involved in anti-parasitic immune responses and frequently also allergic reactions
- The surface expression of CD200R3 decreases when basophils are activated by ligating their CD16 and CD32
- Remarkably, most of this downregulation is observed within the first 30 minutes after activation
- Unlike CD200R, CD200R3 does not bind CD200.
Compare Similar Items
Show Difference
Antigen: CD200R3
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: 4733401I18Rik; 4833409J19Rik; AI505817; BB106877; CD200 cell surface glycoprotein receptor 3; CD200 cell surface glycoprotein receptor-like 3; CD200 cell surface glycoprotein receptor-like b; CD200 receptor 3; CD200 receptor-like 3; Cd200r3; CD200RLb; cell surface glycoprotein CD200 receptor 3; Cell surface glycoprotein OX2 receptor 3; mCD200RLb
Gene Symbols: CD200R3
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Clone: Ba13
Applications: Flow Cytometry
Conjugate: PE-Cyanine7
Host Species: Rat
Target Species: Mouse
Gene Accession No.: Q5UKY4
Gene ID (Entrez): 74603
Immunogen: Mouse primary basophils
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: CD200R3
Antigen:
CD200R3
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
4733401I18Rik; 4833409J19Rik; AI505817; BB106877; CD200 cell surface glycoprotein receptor 3; CD200 cell surface glycoprotein receptor-like 3; CD200 cell surface glycoprotein receptor-like b; CD200 receptor 3; CD200 receptor-like 3; Cd200r3; CD200RLb; cell surface glycoprotein CD200 receptor 3; Cell surface glycoprotein OX2 receptor 3; mCD200RLb
Gene Symbols:
CD200R3
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
Ba13
Applications:
Flow Cytometry
Conjugate:
PE-Cyanine7
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
Q5UKY4
Gene ID (Entrez):
74603
Immunogen:
Mouse primary basophils
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
CD200R3
Antigen: CD137 (4-1BB)
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias: 4 1 BB; 41BB; 4-1BB; 4-1BB ligand receptor; A930040I11Rik; AA408498; AI325004; Cd137; CD137 antigen; CDw137; EGK_00183; homolog of mouse 4-1BB; ILA; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; Ly63; receptor protein 4-1BB; s4 1 BB; s4 1BB; s41BB; sCD137; secreted CD137 antigen; soluble 4 1 BB; soluble 4 1BB; soluble 41BB; soluble CD137; T cell antigen ILA; T-cell antigen 4-1BB; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNF receptor superfamily member 9; TNFRSF9; Tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9
Gene Symbols: TNFRSF9
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Clone: 4B4 (4B4-1)
Applications: Flow Cytometry
Conjugate: Alexa Fluor 700
Host Species: Mouse
Target Species: Human
Gene Accession No.: Q07011
Gene ID (Entrez): 3604
Immunogen: __
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: TNFRSF9
Antigen:
CD137 (4-1BB)
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
4 1 BB; 41BB; 4-1BB; 4-1BB ligand receptor; A930040I11Rik; AA408498; AI325004; Cd137; CD137 antigen; CDw137; EGK_00183; homolog of mouse 4-1BB; ILA; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; Ly63; receptor protein 4-1BB; s4 1 BB; s4 1BB; s41BB; sCD137; secreted CD137 antigen; soluble 4 1 BB; soluble 4 1BB; soluble 41BB; soluble CD137; T cell antigen ILA; T-cell antigen 4-1BB; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNF receptor superfamily member 9; TNFRSF9; Tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9
Gene Symbols:
TNFRSF9
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
4B4 (4B4-1)
Applications:
Flow Cytometry
Conjugate:
Alexa Fluor 700
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
Q07011
Gene ID (Entrez):
3604
Immunogen:
__
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
TNFRSF9
Antigen: CD45
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220
Gene Symbols: PTPRC
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Product Type: Antibody
Clone: 30-F11
Applications: Flow Cytometry, Immunohistochemistry (Paraffin)
Conjugate: Alexa Fluor 488
Host Species: Rat
Target Species: Mouse
Gene Accession No.: P06800
Gene ID (Entrez): 19264
Immunogen: __
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: PTPRC
Antigen:
CD45
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220
Gene Symbols:
PTPRC
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
30-F11
Applications:
Flow Cytometry, Immunohistochemistry (Paraffin)
Conjugate:
Alexa Fluor 488
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
P06800
Gene ID (Entrez):
19264
Immunogen:
__
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
PTPRC
Antigen: CD16
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias: 4833442P21Rik; CD16; CD16-2; CD16A; CD16a antigen; CD16b; CD32; CD32 receptor 2; CDw32; cytolytic trigger molecule G7; Fc fragment of IgG intermediate affinity IV receptor; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG receptor IIIa; Fc fragment of IgG receptor IIIb; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III; Fc gamma receptor IIIa; Fc gamma receptor III-A; Fc gamma receptor IIIb; Fc gamma RIIIa; Fc gammaRIV; Fc receptor, IgG, low affinity III; Fc receptor, IgG, low affinity IV; Fc receptor-like 3; Fcg receptor III; FCG2; FCG3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RII-b; Fc-gamma RII-c; fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; fc-gamma RIIIb; fc-gamma RIII-beta; Fc-gamma-RIIb; Fc-gamma-RIIc; FcgammaRIII; FcgammaRIII a.1; FcgammaRIII a.2; FcgammaRIII a.3; FcgammaRIIIA; FcgammaRIV; FCGR2; FCGR2A; FCGR2B; FCGR2C; FCGR3; Fcgr3a; FCGR3B; Fcgr4; FCGRIII; FcgRIV; FcR-10; FcRII-b; FcRII-c; FcRIII; FCRIIIA; FCRIIIb; Fcrl3; IGFR3; IgG Fc gamma receptor III; igG Fc receptor III; IgG Fc receptor III-1; igG Fc receptor III-2; IMD20; immunoglobulin G Fc receptor III; LOC100911825; Low affinity immunoglobulin gamma Fc region receptor III; low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; low affinity immunoglobulin gamma Fc region receptor III-like; Low affinity immunoglobulin gamma Fc region receptor IV; neutrophil-specific antigen NA; RP11-5K23.1; transmembrane receptor FcgammaRIII-X
Gene Symbols: Fcgr3, FCGR3A, FCGR3B
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Clone: 3G8
Applications: Flow Cytometry
Conjugate: APC-eFluor 780
Host Species: Mouse
Target Species: Baboon, Chimpanzee, Cynomolgus Monkey, Human, Non-human Primate, Rhesus Monkey
Gene Accession No.: A3RFZ7, O75015, P08637
Gene ID (Entrez): 2214, 2215, 720006
Immunogen: __
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: FCGR3A
Antigen:
CD16
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
4833442P21Rik; CD16; CD16-2; CD16A; CD16a antigen; CD16b; CD32; CD32 receptor 2; CDw32; cytolytic trigger molecule G7; Fc fragment of IgG intermediate affinity IV receptor; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG receptor IIIa; Fc fragment of IgG receptor IIIb; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III; Fc gamma receptor IIIa; Fc gamma receptor III-A; Fc gamma receptor IIIb; Fc gamma RIIIa; Fc gammaRIV; Fc receptor, IgG, low affinity III; Fc receptor, IgG, low affinity IV; Fc receptor-like 3; Fcg receptor III; FCG2; FCG3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RII-b; Fc-gamma RII-c; fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; fc-gamma RIIIb; fc-gamma RIII-beta; Fc-gamma-RIIb; Fc-gamma-RIIc; FcgammaRIII; FcgammaRIII a.1; FcgammaRIII a.2; FcgammaRIII a.3; FcgammaRIIIA; FcgammaRIV; FCGR2; FCGR2A; FCGR2B; FCGR2C; FCGR3; Fcgr3a; FCGR3B; Fcgr4; FCGRIII; FcgRIV; FcR-10; FcRII-b; FcRII-c; FcRIII; FCRIIIA; FCRIIIb; Fcrl3; IGFR3; IgG Fc gamma receptor III; igG Fc receptor III; IgG Fc receptor III-1; igG Fc receptor III-2; IMD20; immunoglobulin G Fc receptor III; LOC100911825; Low affinity immunoglobulin gamma Fc region receptor III; low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; low affinity immunoglobulin gamma Fc region receptor III-like; Low affinity immunoglobulin gamma Fc region receptor IV; neutrophil-specific antigen NA; RP11-5K23.1; transmembrane receptor FcgammaRIII-X
Gene Symbols:
Fcgr3, FCGR3A, FCGR3B
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
3G8
Applications:
Flow Cytometry
Conjugate:
APC-eFluor 780
Host Species:
Mouse
Target Species:
Baboon, Chimpanzee, Cynomolgus Monkey, Human, Non-human Primate, Rhesus Monkey
Gene Accession No.:
A3RFZ7, O75015, P08637
Gene ID (Entrez):
2214, 2215, 720006
Immunogen:
__
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
FCGR3A