Paramyxovirus SV5 Pk Antibody (SV5-Pk1) - BSA Free, Novus Biologicals™
Mouse Monoclonal Antibody has been used in 23 publications
Manufacturer: Fischer Scientific
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Antigen
Paramyxovirus SV5 Pk
Concentration
1.0 mg/mL
Applications
Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, ELISA, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Epitope Tags, V5 Epitope Tags
Gene Alias
Simian virus 5; SPV5gp2; V protein
Isotype
IgG2a
Purification Method
Protein G purified
Test Specificity
This product recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. NB100-62264 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro (ile pro asn pro leu leu gly leu asp) ser thr. (9 amino acid epitope is in parenthesis)
Clone
SV5-Pk1
Dilution
Western Blot 1:1000-1:5000, Flow Cytometry 1:10-1:1000, ELISA 1:1000-1:5000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500RadioImmunoassay 1:100-1:2000, Immunohistochemistry-Frozen 1:10-1:500, Radioimmunoassay 1:100-1:2000, Sandwich ELISA
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Virus, Mouse
Immunogen
Mice were infected with the paramyxovirus SV5, Simian-Virus 5
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Description
- Paramyxovirus SV5 Pk Monoclonal specifically detects Paramyxovirus SV5 Pk in Mouse, Virus samples
- It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen, Radioimmunoassay, Sandwich ELISA.
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Antigen: Paramyxovirus SV5 Pk
Concentration: 1.0 mg/mL
Applications: Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, ELISA, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Epitope Tags, V5 Epitope Tags
Gene Alias: Simian virus 5; SPV5gp2; V protein
Isotype: IgG2a
Purification Method: Protein G purified
Test Specificity: This product recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. NB100-62264 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro (ile pro asn pro leu leu gly leu asp) ser thr. (9 amino acid epitope is in parenthesis)
Clone: SV5-Pk1
Dilution: Western Blot 1:1000-1:5000, Flow Cytometry 1:10-1:1000, ELISA 1:1000-1:5000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500RadioImmunoassay 1:100-1:2000, Immunohistochemistry-Frozen 1:10-1:500, Radioimmunoassay 1:100-1:2000, Sandwich ELISA
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Virus, Mouse
Immunogen: Mice were infected with the paramyxovirus SV5, Simian-Virus 5
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen: LBX1
Concentration: __
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: __
Gene Alias: homeobox, HPX6, HPX-6, ladybird homeobox 1, ladybird homeobox homolog 1, ladybird homeobox homolog 1 (Drosophila), Ladybird homeobox protein homolog 1, LBX1Hlady bird-like homeobox, transcription factor LBX1, transcription factor similar to D. melanogaster homeodomain protein lady birdlate
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: Lbx1 (Ladybird). By Western Blot the antibody recognizes a band at ∼30 kDa on mouse spinal cord lysate.
Clone: __
Dilution: Western Blot 1:500-1:1000
Classification: Polyclonal
Form: __
Regulatory Status: RUO
Target Species: Mouse
Immunogen: Synthetic peptide.
Primary or Secondary: Primary
Content And Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Antigen: ABCG2/CD338
Concentration: LYOPH
Applications: CyTOF, Flow Cytometry, Immunohistochemistry (Paraffin), Immunohistochemistry
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: ABC Transporters, Cancer, Hematopoietic Stem Cell Markers, Neuronal Cell Markers, Neuronal Stem Cell Markers, Stem Cell Markers, Stem Cells
Gene Alias: ABC transporter, ABC15, ABCPMGC102821, ATP-binding cassette sub-family G member 2, ATP-binding cassette transporter G2, ATP-binding cassette, sub-family G (WHITE), member 2, BCRP1, BCRPMRX, BMDP, Breast cancer resistance protein, CD338, CD338 antigen, CDw338, EST157481, mitoxantrone resistance protein, Mitoxantrone resistance-associated protein, MXRMXR1, placenta specific MDR protein, Placenta-specific ATP-binding cassette transporter
Isotype: IgG2
Purification Method: Protein G purified
Test Specificity: This was selected for its ability to detect human ABCG2 by flow cytometry and immunocytochemistry.
Clone: MM0047-2J39
Dilution: Flow Cytometry 1:100-1000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1000, CyTOF-ready
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Immunogen: human recombinant rHABCG2 protein
Primary or Secondary: Primary
Content And Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
