Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Siglec-1/CD169
0.5 mg/mL
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Frozen)
Unconjugated
Mouse
Rat
6614
Rat Spleen cell homogenate
Primary
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
ED3
Flow Cytometry 1:500, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Frozen 1:50-1:250
Monoclonal
Purified
RUO
CD169, FLJ00051, sialic acid binding Ig-like lectin 1, sialoadhesin, sialoadhesin
SIGLEC1
IgG2a
Protein A purified
NB100-65301 recognizes the rat CD169 cell surface antigen, a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.