Home Products Antibodies Primary Antibodies 7200587
7200587

SerpinB9 Antibody (7D8) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

SerpinB9

Concentration

1.0 mg/mL

Applications

Immunohistochemistry (Frozen), Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin), Flow Cytometry

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Apoptosis, Immunology

Gene Accession No.

P50453

Gene ID (Entrez)

5272

Immunogen

Recombinant PI-9 produced in P. pastoris.

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Clone

7D8

Dilution

Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:100, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50, Immunohistochemistry-Frozen 1:10-1:500

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Mouse (Negative), Porcine (Negative), Human

Gene Alias

CAP-3, CAP3PI-9, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, PI9protease inhibitor 9 (ovalbumin type), serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 9, serpin B9, serpin peptidase inhibitor, clade B (ovalbumin), member 9, serpin peptidase inhibitor, clade B, member 9

Gene Symbols

SERPINB9

Isotype

IgG1

Purification Method

Protein G purified

Test Specificity

NB100-64359 is specific for human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a 42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Clone 7D8 detects a band of approximately 42kDa under reducing conditions. Clone 7D8 is suitable for use in indirect Immunofluorescence (2).

Description

  • SerpinB9 Monoclonal specifically detects SerpinB9 in Human, Mouse (Negative), Porcine (Negative) samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Compare Similar Items

Show Difference

Img

Fischer Scientific

7200587

Mouse Monoclonal Antibody...

Antigen:
SerpinB9
Concentration:
1.0 mg/mL
Applications:
Immunohistochemistry (Frozen), Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin), Flow Cytometry
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Apoptosis, Immunology
Gene Accession No.:
P50453
Gene ID (Entrez):
5272
Immunogen:
Recombinant PI-9 produced in P. pastoris.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone:
7D8
Dilution:
Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:100, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50, Immunohistochemistry-Frozen 1:10-1:500
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse (Negative), Porcine (Negative), Human
Gene Alias:
CAP-3, CAP3PI-9, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, PI9protease inhibitor 9 (ovalbumin type), serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 9, serpin B9, serpin peptidase inhibitor, clade B (ovalbumin), member 9, serpin peptidase inhibitor, clade B, member 9
Gene Symbols:
SERPINB9
Isotype:
IgG1
Purification Method:
Protein G purified
Test Specificity:
NB100-64359 is specific for human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a 42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Clone 7D8 detects a band of approximately 42kDa under reducing conditions. Clone 7D8 is suitable for use in indirect Immunofluorescence (2).

Img

Fischer Scientific

7200588

Mouse Monoclonal Antibody has been ...

Antigen:
__
Concentration:
1.0 mg/mL
Applications:
Immunohistochemistry (Frozen), Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry (Paraffin), ELISA
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
__
Gene Accession No.:
P18072
Gene ID (Entrez):
__
Immunogen:
From Texas strain
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone:
1331
Dilution:
ELISA 1:200-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen 1:100-1:500
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Virus, Ferret
Gene Alias:
Flu
Gene Symbols:
NP
Isotype:
IgG2a
Purification Method:
Protein A purified
Test Specificity:
Specific for the nucleoprotein of numerous strains and hence gives a 'nuclear' staining in IF.

Img

Fischer Scientific

7200589

Mouse Monoclonal Antibody has been ...

Antigen:
Peripherin
Concentration:
1 mg/mL
Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
__
Gene Accession No.:
__
Gene ID (Entrez):
5630
Immunogen:
Recombinant rat Peripherin purified from E. coli. [UniProtNo. P21807]
Primary or Secondary:
Primary
Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone:
8G2
Dilution:
Western Blot 1:5000, Immunohistochemistry 1:500, Immunocytochemistry/Immunofluorescence 1:500
Classification:
Monoclonal
Form:
__
Regulatory Status:
__
Target Species:
Human, Mouse, Rat, Porcine, Bovine
Gene Alias:
Neurofilament 4, neurofilament 4 (57kD), peripherin, PRPH1NEF4
Gene Symbols:
PRPH
Isotype:
IgG1
Purification Method:
__
Test Specificity:
__

Img

Fischer Scientific

7200593

Sheep Polyclonal Antibody has been ...

Antigen:
HCN1
Concentration:
LYOPH
Applications:
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Conjugate:
Unconjugated
Host Species:
Sheep
Research Discipline:
__
Gene Accession No.:
O60741
Gene ID (Entrez):
348980
Immunogen:
A synthetic peptide from cytoplasmic region of human HCN1 conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in many species including mouse and rat.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone:
__
Dilution:
__
Classification:
Polyclonal
Form:
Antisera
Regulatory Status:
RUO
Target Species:
Mouse, Human, Rat
Gene Alias:
BCNG1, BCNG-1brain cyclic nucleotide gated channel 1, Brain cyclic nucleotide-gated channel 1, HAC-2, hyperpolarization activated cyclic nucleotide-gated potassium channel 1, potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Gene Symbols:
HCN1
Isotype:
IgG
Purification Method:
Unpurified
Test Specificity:
HCN1.