7200816
E. Coli J5 LPS Antibody, Novus Biologicals™
Sheep Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
E. Coli J5 LPS
Applications
ELISA
Conjugate
Unconjugated
Host Species
Sheep
Target Species
Bacteria
Gene Alias
Escherichia coli
Isotype
IgG
Purification Method
Unpurified
Test Specificity
NB100-62551 recognizes the J5 mutant of E. coli 0:111 which lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The 55 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).
Dilution
ELISA 1:100-1:2000
Classification
Polyclonal
Form
Antisera
Regulatory Status
RUO
Formulation
Whole antisera with 0.09% Sodium Azide
Immunogen
Heat inactivated Escherichia coli J5 (Rc mutant; ATCC)
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Related Products
Description
- E
- Coli J5 LPS Polyclonal specifically detects E
- Coli J5 LPS in Bacteria samples
- It is validated for ELISA.
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Antigen: E. Coli J5 LPS
Applications: ELISA
Conjugate: Unconjugated
Host Species: Sheep
Target Species: Bacteria
Gene Alias: Escherichia coli
Isotype: IgG
Purification Method: Unpurified
Test Specificity: NB100-62551 recognizes the J5 mutant of E. coli 0:111 which lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The 55 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).
Dilution: ELISA 1:100-1:2000
Classification: Polyclonal
Form: Antisera
Regulatory Status: RUO
Formulation: Whole antisera with 0.09% Sodium Azide
Immunogen: Heat inactivated Escherichia coli J5 (Rc mutant; ATCC)
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen:
E. Coli J5 LPS
Applications:
ELISA
Conjugate:
Unconjugated
Host Species:
Sheep
Target Species:
Bacteria
Gene Alias:
Escherichia coli
Isotype:
IgG
Purification Method:
Unpurified
Test Specificity:
NB100-62551 recognizes the J5 mutant of E. coli 0:111 which lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The 55 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).
Dilution:
ELISA 1:100-1:2000
Classification:
Polyclonal
Form:
Antisera
Regulatory Status:
RUO
Formulation:
Whole antisera with 0.09% Sodium Azide
Immunogen:
Heat inactivated Escherichia coli J5 (Rc mutant; ATCC)
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen: VASP (p Ser238)
Applications: Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: __
Gene Alias: vasodilator-stimulated phosphoprotein
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: __
Dilution: __
Classification: Polyclonal
Form: __
Regulatory Status: RUO
Formulation: __
Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human VASP around the phosphorylation site of serine 238 (K-V-SP-K-Q).
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen:
VASP (p Ser238)
Applications:
Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
__
Gene Alias:
vasodilator-stimulated phosphoprotein
Isotype:
IgG
Purification Method:
Affinity Purified
Test Specificity:
__
Dilution:
__
Classification:
Polyclonal
Form:
__
Regulatory Status:
RUO
Formulation:
__
Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human VASP around the phosphorylation site of serine 238 (K-V-SP-K-Q).
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen: P-Selectin/CD62P
Applications: Immunoprecipitation, Immunohistochemistry, Immunohistochemistry (Frozen), Flow Cytometry
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Goat, Human, Sheep, Rat
Gene Alias: CD62P antigen, FLJ45155, PADGEMantigen CD62), selectin P (granule membrane protein 140kDa, antigen CD62)
Isotype: IgG1
Purification Method: Protein G purified
Test Specificity: NB100-65391 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells. Clone Psel.KO.2.12 is reported to inhibit P-selectin-dependent adhesion between HL60 cells and P-selectin transfected COS cells.
Dilution: Flow Cytometry 1:50-1:100, Immunohistochemistry 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: __
Immunogen: P-Selectin transfected 300.19 cells.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen:
P-Selectin/CD62P
Applications:
Immunoprecipitation, Immunohistochemistry, Immunohistochemistry (Frozen), Flow Cytometry
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Goat, Human, Sheep, Rat
Gene Alias:
CD62P antigen, FLJ45155, PADGEMantigen CD62), selectin P (granule membrane protein 140kDa, antigen CD62)
Isotype:
IgG1
Purification Method:
Protein G purified
Test Specificity:
NB100-65391 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells. Clone Psel.KO.2.12 is reported to inhibit P-selectin-dependent adhesion between HL60 cells and P-selectin transfected COS cells.
Dilution:
Flow Cytometry 1:50-1:100, Immunohistochemistry 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
__
Immunogen:
P-Selectin transfected 300.19 cells.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen: P-Selectin/CD62P
Applications: Immunoprecipitation, Immunohistochemistry, Immunohistochemistry (Frozen), Flow Cytometry
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Goat, Human, Sheep, Rat
Gene Alias: CD62P antigen, FLJ45155, PADGEMantigen CD62), selectin P (granule membrane protein 140kDa, antigen CD62)
Isotype: IgG1
Purification Method: Protein G purified
Test Specificity: NB100-65391 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells. Clone Psel.KO.2.12 is reported to inhibit P-selectin-dependent adhesion between HL60 cells and P-selectin transfected COS cells.
Dilution: Flow Cytometry 1:50-1:100, Immunohistochemistry 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: __
Immunogen: P-Selectin transfected 300.19 cells.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen:
P-Selectin/CD62P
Applications:
Immunoprecipitation, Immunohistochemistry, Immunohistochemistry (Frozen), Flow Cytometry
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Goat, Human, Sheep, Rat
Gene Alias:
CD62P antigen, FLJ45155, PADGEMantigen CD62), selectin P (granule membrane protein 140kDa, antigen CD62)
Isotype:
IgG1
Purification Method:
Protein G purified
Test Specificity:
NB100-65391 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells. Clone Psel.KO.2.12 is reported to inhibit P-selectin-dependent adhesion between HL60 cells and P-selectin transfected COS cells.
Dilution:
Flow Cytometry 1:50-1:100, Immunohistochemistry 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
__
Immunogen:
P-Selectin transfected 300.19 cells.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.