Rat Monoclonal Antibody has been used in 1 publication
Manufacturer: Fischer Scientific
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ER-HR3
1.0 mg/mL
Immunohistochemistry (Frozen), Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Unconjugated
Rat
Mouse
Adherent F1 (CBAxBL) bone marrow stormal cells.
Primary
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
ER-HR3
Flow Cytometry 1:10-1:50, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Monoclonal
Purified
RUO
Hematopoiesis related macrophage
IgG2c
Protein G purified
NB100-64931 recognizes the murine antigen ER-HR3. ER-HR3 is a cell surface antigen which is expressed by Langerhans cells in epithelium, a subset of mature macrophages and dendritic cells that are located predominantly in haematopoietic and lymphoid organs. Initial studies of ER-HR3 demonstrated expression on peripheral blood monocytes but recent studies suggest that expression is actually very low. During foetal development, ER-HR3 positive cells are localised to haemapoietic islands and are often associated with erythroid progenitor cells. The functions of the ER-HR3 antigen have not been established but reports suggest that the antigen may be involved in adult erythropoiesis and in the regulation of the immune response. Clone ER-HR3 does not inhibit T cell proliferation in antigen-specific T-cell proliferation studies. Clone ER-HR3 recognizes two proteins of approximately 69kD and 55kD under non-reducing conditions.