CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), eBioscience™, Invitrogen™

Description

• Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1)
• CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells
• CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+
• The CD90+CD34+ population is enriched for cells capable of long-term culture
• CD90 is involved in regulation of adhesion and signal transduction by T cells
• Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections
• Applications Tested: This eBio5E10 (5E10) antibody has been tested by flow cytometric analysis of human erythroleukemia (HEL) cells
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family
• CD90 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts
• CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain
• CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons
• Structural study of CD90 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor
• Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.