CD62E (E-selectin) Monoclonal Antibody (P2H3), eBioscience™, Invitrogen™

Description

• Description: The P2H3 monoclonal antibody reacts with human CD62E, a 97-115 kDa member of the selectin family
• CD62E, also known as E-selectin or endothelial-leukocyte adhesion molecule-1 (ELAM-1) is an adhesion molecule expressed by endothelial cells upon stimulation with cytokines including TNFalpha and IL-1beta
• Induced expression of CD62E during inflammatory conditions is thought to mediate leukocyte rolling including the initial interaction of neutrophils with endothelium
• The P2H3 monoclonal antibody also inhibits cellular adhesion to cytokine-activated endothelial cells
• Applications Reported: This P2H3 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining
• Applications Tested: This P2H3 antibody has been tested by flow cytometric analysis of TNF alpha-actiavted Human Umbilical Vein Endothelial Cells (HUVEC)
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes
• In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation
• This response is immediate and involves a cascade of adhesion receptors
• At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade
• Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium
• E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines
• In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade
• E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.