MAVS Monoclonal Antibody (ABM28H9), APC, eBioscience™, Invitrogen™

Description

• Description: This ABM28H9 monoclonal antibody recognizes human MAVS, a mitochondrial membrane protein also known as VISA, CARDIF or IPS-1
• Applications Reported: This ABM18H9 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This ABM18H9 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of HEL cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol
• Please refer to Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins located at www.thermofisher.com/flowprotocols
• This may be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser Two distinct signaling pathways activate the host innate immunity against viral infection
• One pathway is reliant on members of the Toll-like receptor (TLR) family while the other uses the RNA helicase RIG-I as a receptor for intracellular viral double-stranded RNA as a trigger for the immune response
• MAVS is a mitochondrial membrane protein that was identified as a critical component in the IFN beta signaling pathways that recruits IRF-3 to RIG-I, leading to its activation and that of NF-kappa-B
• MAVS is also thought to interact with other components of the innate immune pathway such as the TLR adapter protein TRIF, TRAF2 and TRAF6
• MAVS also interacts with the IKK-alpha, IKK-beta and IKK-iota kinases through its C-terminal region
• Cleavage of this region by the Hepatitis C virus (HCV) protease allows HCV to escape the host immune system
• Multiple isoforms of MAVS are known to exist.