7218754
CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), APC-eFluor™ 780, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD235a (Glycophorin A)
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias
AI853584; CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A; glycophorin A (MN blood group); glycophorin A (MNS blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GPSAT; Gypa; HGNC:4702; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN; MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR; sialoglycoprotein alpha
Gene Symbols
GYPA
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
GYPA
Clone
HIR2 (GA-R2)
Applications
Flow Cytometry
Conjugate
APC-eFluor 780
Host Species
Mouse
Target Species
Human
Gene Accession No.
P02724
Gene ID (Entrez)
2993
Isotype
IgG2b κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The HIR2 monoclonal antibody reacts with human glycophorin A, sialoglycoproteins expressed by erythroid precursors and mature circulating red cells
- Applications Reported: This HIR2 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This HIR2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- This may be used at 5 μL (0.03 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm)
- Please make sure that your instrument is capable of detecting this fluorochrome
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
- Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
- Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser CD235a (Glycophorins A, GPA) is a single pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells
- CD235a is the carrier of blood group M and N specificities
- CD235a provides cells with a large mucin-like that may serve as a barrier to cell fusion, minimizing aggregation between red blood cells in the circulation
- CD235a has been shown to act as a receptor for Sandei virus, parvovirus, and Hsa, an Streptococcus adhesin.
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Antigen: CD235a (Glycophorin A)
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias: AI853584; CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A; glycophorin A (MN blood group); glycophorin A (MNS blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GPSAT; Gypa; HGNC:4702; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN; MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR; sialoglycoprotein alpha
Gene Symbols: GYPA
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: GYPA
Clone: HIR2 (GA-R2)
Applications: Flow Cytometry
Conjugate: APC-eFluor 780
Host Species: Mouse
Target Species: Human
Gene Accession No.: P02724
Gene ID (Entrez): 2993
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD235a (Glycophorin A)
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
AI853584; CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A; glycophorin A (MN blood group); glycophorin A (MNS blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GPSAT; Gypa; HGNC:4702; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN; MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR; sialoglycoprotein alpha
Gene Symbols:
GYPA
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
GYPA
Clone:
HIR2 (GA-R2)
Applications:
Flow Cytometry
Conjugate:
APC-eFluor 780
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P02724
Gene ID (Entrez):
2993
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD3
Concentration: 1 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: TBS with no preservative; pH 8.0
Gene Alias: 4930549J05Rik; A430104F18Rik; AI504783; antigen CD3D, delta polypeptide (TiT3 complex); antigen CD3E, epsilon polypeptide (TiT3 complex); antigen CD3G, gamma polypeptide; antigen CD3Z, zeta polypeptide; AW552088; Cd247; CD247 antigen; CD247 antigen, zeta subunit; Cd247 molecule; CD3; CD3 antigen delta chain; CD3 antigen delta polypeptide; CD3 antigen gamma chain; CD3 antigen, delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 delta; CD3 epsilon; CD3 epsilon chain; CD3 epsilon subunit; CD3 epsilon subunit precursor; CD3 gamma-chain; CD3 glycoprotein; CD3 glycoprotein precursor; CD3 molecule delta polypeptide; CD3 molecule, delta; CD3 molecule, epsilon; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma; CD3 molecule, gamma polypeptide; CD3 protein; CD3 TCR complex; CD3 type I transmembrane glycoprotein; CD3 type I transmembrane glycoprotein precursor; CD3 zeta chain; Cd3d; CD3D antigen delta; CD3D antigen, delta polypeptide (TiT3 complex); CD3d molecule; CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; Cd3e; CD3E antigen, epsilon polypeptide; CD3E antigen, epsilon polypeptide (TiT3 complex); CD3e molecule; CD3e molecule, epsilon (CD3-TCR complex); CD3epsilon; CD3-epsilon; Cd3-eta; Cd3g; CD3G antigen, gamma polypeptide; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule; CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3-GAMMA; Cd3h; CD3Q; Cd3z; CD3Z antigen, zeta polypeptide (TiT3 complex); Cd3zeta; Cd3-zeta; CD3zeta chain; CD3-zeta/eta; Ctg3; Ctg-3; FLJ18683; IMD17; IMD18; IMD19; IMD25; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T3/TCR complex; T3d; T3e; T3g; T3Z; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor CD3 epsilon chain; T-cell receptor CD3 epsilon subunit; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell receptor zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T-cell surface protein; TcR CD3 delta-chain; TcR CD3 gamma-chain; TCR zeta chain; TCR zeta chain subunit; TCRE; Tcrk; TCRZ; TCRzeta; TiT3 complex; type I transmembrane protein; T-cell surface molecule CD3
Gene Symbols: Cd247, Cd3d, CD3E, CD3g
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: __
Clone: OKT3
Applications: Flow Cytometry, Functional Assay
Conjugate: Functional Grade
Host Species: Mouse
Target Species: Human
Gene Accession No.: P04234, P07766, P09693, P20963
Gene ID (Entrez): 915, 916, 917, 919
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD3
Concentration:
1 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
TBS with no preservative; pH 8.0
Gene Alias:
4930549J05Rik; A430104F18Rik; AI504783; antigen CD3D, delta polypeptide (TiT3 complex); antigen CD3E, epsilon polypeptide (TiT3 complex); antigen CD3G, gamma polypeptide; antigen CD3Z, zeta polypeptide; AW552088; Cd247; CD247 antigen; CD247 antigen, zeta subunit; Cd247 molecule; CD3; CD3 antigen delta chain; CD3 antigen delta polypeptide; CD3 antigen gamma chain; CD3 antigen, delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 delta; CD3 epsilon; CD3 epsilon chain; CD3 epsilon subunit; CD3 epsilon subunit precursor; CD3 gamma-chain; CD3 glycoprotein; CD3 glycoprotein precursor; CD3 molecule delta polypeptide; CD3 molecule, delta; CD3 molecule, epsilon; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma; CD3 molecule, gamma polypeptide; CD3 protein; CD3 TCR complex; CD3 type I transmembrane glycoprotein; CD3 type I transmembrane glycoprotein precursor; CD3 zeta chain; Cd3d; CD3D antigen delta; CD3D antigen, delta polypeptide (TiT3 complex); CD3d molecule; CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; Cd3e; CD3E antigen, epsilon polypeptide; CD3E antigen, epsilon polypeptide (TiT3 complex); CD3e molecule; CD3e molecule, epsilon (CD3-TCR complex); CD3epsilon; CD3-epsilon; Cd3-eta; Cd3g; CD3G antigen, gamma polypeptide; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule; CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3-GAMMA; Cd3h; CD3Q; Cd3z; CD3Z antigen, zeta polypeptide (TiT3 complex); Cd3zeta; Cd3-zeta; CD3zeta chain; CD3-zeta/eta; Ctg3; Ctg-3; FLJ18683; IMD17; IMD18; IMD19; IMD25; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T3/TCR complex; T3d; T3e; T3g; T3Z; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor CD3 epsilon chain; T-cell receptor CD3 epsilon subunit; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell receptor zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T-cell surface protein; TcR CD3 delta-chain; TcR CD3 gamma-chain; TCR zeta chain; TCR zeta chain subunit; TCRE; Tcrk; TCRZ; TCRzeta; TiT3 complex; type I transmembrane protein; T-cell surface molecule CD3
Gene Symbols:
Cd247, Cd3d, CD3E, CD3g
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
__
Clone:
OKT3
Applications:
Flow Cytometry, Functional Assay
Conjugate:
Functional Grade
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P04234, P07766, P09693, P20963
Gene ID (Entrez):
915, 916, 917, 919
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD79b
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: AGM6; B29; B-cell antigen receptor complex-associated protein beta chain; B-cell-specific glycoprotein B29; CD79; CD79b; CD79B antigen; CD79b molecule; Cd79b molecule, immunoglobulin-associated beta; Ig beta; IGB; Igbeta; Ig-beta; Immunoglobulin-associated B29 protein; immunoglobulin-associated beta; RP23-418O11.7
Gene Symbols: Cd79b
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: Cd79b
Clone: CB3-1
Applications: Flow Cytometry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human
Gene Accession No.: P40259
Gene ID (Entrez): 974
Isotype: IgG1
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD79b
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
AGM6; B29; B-cell antigen receptor complex-associated protein beta chain; B-cell-specific glycoprotein B29; CD79; CD79b; CD79B antigen; CD79b molecule; Cd79b molecule, immunoglobulin-associated beta; Ig beta; IGB; Igbeta; Ig-beta; Immunoglobulin-associated B29 protein; immunoglobulin-associated beta; RP23-418O11.7
Gene Symbols:
Cd79b
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
Cd79b
Clone:
CB3-1
Applications:
Flow Cytometry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P40259
Gene ID (Entrez):
974
Isotype:
IgG1
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD79b
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: AGM6; B29; B-cell antigen receptor complex-associated protein beta chain; B-cell-specific glycoprotein B29; CD79; CD79b; CD79B antigen; CD79b molecule; Cd79b molecule, immunoglobulin-associated beta; Ig beta; IGB; Igbeta; Ig-beta; Immunoglobulin-associated B29 protein; immunoglobulin-associated beta; RP23-418O11.7
Gene Symbols: Cd79b
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: Cd79b
Clone: CB3-1
Applications: Flow Cytometry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human
Gene Accession No.: P40259
Gene ID (Entrez): 974
Isotype: IgG1
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD79b
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
AGM6; B29; B-cell antigen receptor complex-associated protein beta chain; B-cell-specific glycoprotein B29; CD79; CD79b; CD79B antigen; CD79b molecule; Cd79b molecule, immunoglobulin-associated beta; Ig beta; IGB; Igbeta; Ig-beta; Immunoglobulin-associated B29 protein; immunoglobulin-associated beta; RP23-418O11.7
Gene Symbols:
Cd79b
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
Cd79b
Clone:
CB3-1
Applications:
Flow Cytometry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P40259
Gene ID (Entrez):
974
Isotype:
IgG1
Purification Method:
Affinity chromatography
Product Type:
Antibody