CD185 (CXCR5) Monoclonal Antibody (MU5UBEE), eBioscience™, Invitrogen™

Description

• The MU5UBEE MAb reacts with Human CD185
• CD185, which is also known as C-X-C chemokine receptor 5 (CXCR5) and Burkitt lymphoma receptor 1 (BLR1), is a seven transmembrane G protein-coupled receptor originally identified in Burkitt's lymphoma
• In peripheral blood, CXCR5 is expressed on B cells, CD4+ T cells (but not Th1 or Th2 cells), as well as on a subpopulation of memory (CD45RO+) T cells
• CXCR5+ circulating T cells are in a resting state and migrate to the lymph nodes due to expression of CCR7 and CD62L
• In tonsil, CXCR5 is expressed on nearly all CD4+ cells along with CD45RO and such activation markers as CD69 and ICOS
• Tonsillar CXCR5+ cells have been shown to induce antibody production when co-cultured with B cells, thus supporting their role in providing B cell help
• Furthermore, this chemokine receptor plays a critical role in lymphocyte trafficking, in particular T cell migration into the B cell follicles of germinal centers in response to CXCL13, making CXCR5 an established marker of follicular helper T cells
• CXCR5 (C-X-Chemokine Receptor Type 5, CD185) also known as Burkitt lymphoma receptor 1 (BLR1) belongs to the CXC chemokine receptor family
• CXCR5 is a G protein-coupled seven transmembrane receptor for chemokine BLC
• CXCR5 gene is specifically expressed in Burkitt's lymphoma and lymphatic tissues, such as follicles in lymph nodes, as well as in spleen
• Studies of CXCR5 suggest that it plays an essential role in B cell migration
• CXCR5 expression has been reported in human blood and salivary gland and in animal lymphatic organs, blood, bone marrow, brain, liver, lymph node, skin, spleen, stomach, and tonsil
• ESTs (expressed sequence tags) have been isolated from human brain, germ cell, lung, lymph, and pancreas
• G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist
• All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms
• An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors
• Research studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs
• This &quoteturn-off&quote activity in GPCRs have tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates
• Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which are used in mental health and allergy applications, respectively.