7218838

MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

MHC Class I (H-2Kb)

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K; H2-K; H-2K(B); H-2K(d); H-2K(K); H-2K(Q); H2-K1; histocompatibility 2, K1, K region; K-f; MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen

Gene Symbols

H2-K1

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

H2-K1

Clone

AF6-88.5.5.3

Applications

Flow Cytometry

Conjugate

Alexa Fluor 700

Host Species

Mouse

Target Species

Mouse

Gene Accession No.

P01901, P03991, P04223, P14428

Gene ID (Entrez)

14972

Isotype

IgG2a κ

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen
  • H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8
  • Reactivity to other haplotypes (e.g
  • d, f, j, k, p, q, r, s, u, and v) has not been observed
  • Applications Reported: This AF6-88.5.5.3 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This AF6-88.5.5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes
  • This may be used at less than or equal to 0.5 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm)
  • Most instruments will require a 685 LP mirror and 710/20 band pass filter
  • Please make sure that your instrument is capable of detecting this fluorochrome
  • Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8
  • HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

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7218838

Mouse Monoclonal Antibody...


Antigen:
MHC Class I (H-2Kb)

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K; H2-K; H-2K(B); H-2K(d); H-2K(K); H-2K(Q); H2-K1; histocompatibility 2, K1, K region; K-f; MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen

Gene Symbols:
H2-K1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
H2-K1

Clone:
AF6-88.5.5.3

Applications:
Flow Cytometry

Conjugate:
Alexa Fluor 700

Host Species:
Mouse

Target Species:
Mouse

Gene Accession No.:
P01901, P03991, P04223, P14428

Gene ID (Entrez):
14972

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Concentration:
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Classification:
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Form:
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Regulatory Status:
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Formulation:
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Gene Alias:
AW495831; B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Cd19; CD19 antigen; CD19 molecule; CVID3; differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12

Gene Symbols:
Cd19

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Cd19

Clone:
HIB19

Applications:
Flow Cytometry

Conjugate:
Alexa Fluor 660

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P15391

Gene ID (Entrez):
930

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

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Antibody

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Armenian Hamster Isotype Control...


Antigen:
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Concentration:
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Classification:
__

Form:
Liquid

Regulatory Status:
RUO

Formulation:
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Gene Alias:
IgG; Immunoglobulin G; ImmunoglobulinG

Gene Symbols:
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Primary or Secondary:
Isotype Control (Primary)

Content And Storage:
4° C

Gene:
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Clone:
eBio299Arm

Applications:
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Conjugate:
Functional Grade

Host Species:
Armenian Hamster

Target Species:
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Gene Accession No.:
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Gene ID (Entrez):
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Isotype:
IgG

Purification Method:
Affinity chromatography

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Concentration:
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Classification:
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Form:
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Regulatory Status:
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Formulation:
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Gene Alias:
AI449405; CD11 antigen-like family member C; CD11c; CD11C (p150) alpha polypeptide; complement component 3 receptor 4 subunit; complement receptor 4; Cr4; integrin alpha X; integrin alpha-X; integrin aX; integrin subunit alpha X; integrin, alpha X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); Itgax; Leu M5; leu M5, alpha subunit; leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; leukocyte surface antigen p150,95, alpha subunit; myeloid membrane antigen, alpha subunit; N418; p150 95 integrin alpha chain; RGD1561123; SLEB6

Gene Symbols:
Itgax

Primary or Secondary:
Primary

Content And Storage:
4° C

Gene:
Itgax

Clone:
118/A5

Applications:
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Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P20702

Gene ID (Entrez):
3687

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

Product Type:
Antibody